Alpha glucosidase inhibitory activity of Heliantis annuus, Terminalia catappa, and Garcinia mangostana L.

• Main Authors: Inocentes, Carl Roger V., Naing, Marvin D., Saluria, Julius Victorious A., Amor, Evangeline C.
• Format: text
• Published: Animo Repository 2012
• Subjects: Common sunflower—Analysis; Terminalia catappa —Analysis; Mangosteen—Analysis; Glucose; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/11431
• Institution: De La Salle University

Diabetes mellitus is a metabolic disease wherein glucose molecules are unable to enter the cell due to insufficiency or absence of the insulin hormone. It is among the top ten causes of mortality in the Philippines. By 2023, the Philippines is expected to be among the top ten countries with the highest prevalence of diabetes. Hence, there is an urgent need to address this issue. One of the enzymes that play a key role in controlling blood glucose level is alpha glucosidase. Inhibition of this enzyme will slow down hydrolysis of the cleavage of glucose from disaccharides and oligosaccharides, prolonging the overall carbohydrates digestion time, and thus reducing the rate of glucose absorption. This study evaluated the alpha glucosidase enzyme inhibitory activity of the methanolic extract of Helianthus annuus, Terminalia catappa and Garcinia mangostana L. Enzyme inhibitory activity was observed spectrophotometrically for 30 minutes at pH 6.8 and 37o C using 1-deoxynojirimycin (1-DNJ) and acarbose as the positive control, and p-nitrophenyl-a-D-glucopyranoside as the substrate. Further fractionation of the crude extracts is being done in order to isolate the compounds responsible for the exhibited bioactivity.