Variation of a conserved region in the nitrate reductase gene(NIAD) of selected aspergillus species

Nitrate reductase is a NADH-dependent redox enzyme that is important for nitrate assimilation, together with nitrite reductase. Recent studies have shown that nitrate reductase plays a major role in the biosynthesis of metal nanoparticles through reduction of the metal salt, such as silver nitrate....

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Main Authors: Alba, Laurenzo De Vera, Lagman, Carmen
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Published: Animo Repository 2010
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/12052
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-137492024-02-13T08:27:01Z Variation of a conserved region in the nitrate reductase gene(NIAD) of selected aspergillus species Alba, Laurenzo De Vera Lagman, Carmen Nitrate reductase is a NADH-dependent redox enzyme that is important for nitrate assimilation, together with nitrite reductase. Recent studies have shown that nitrate reductase plays a major role in the biosynthesis of metal nanoparticles through reduction of the metal salt, such as silver nitrate. This research is interested in optimizing the selection of fungi for use in biogenic synthesis of nanoparticles. It is possible that species with the same of minor differences in the niaD sequence may produce similar nanoparticles. A portion of the nitrate reductase gene niaD was amplified from Aspergillus oryzae, A. flavus, A. niger, and A. versicolor obtained from the University of the Philippines Culture Collections. The forward and reverse primers as described by Kitamoto et al (1995) were used, with primers being regarded as conserved for the genus. Of the fungi tested, A. niger did not amplify successfully. The analysis and alignment of the sequences showed large (translated to at least 45 amino acids) differences between any pair of species. Phylogeny analysis using the amplified sequence and homologous sequences of other Aspergillus species showed inconsistencies in distance between the same species. It is therefore recommended that all four species be used in nanoparticle synthesis, as the activity of nitrate reductase can be different in all of these. 2010-01-05T08:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/12052 Faculty Research Work Animo Repository Aspergillus Nitrate Biology
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Aspergillus
Nitrate
Biology
spellingShingle Aspergillus
Nitrate
Biology
Alba, Laurenzo De Vera
Lagman, Carmen
Variation of a conserved region in the nitrate reductase gene(NIAD) of selected aspergillus species
description Nitrate reductase is a NADH-dependent redox enzyme that is important for nitrate assimilation, together with nitrite reductase. Recent studies have shown that nitrate reductase plays a major role in the biosynthesis of metal nanoparticles through reduction of the metal salt, such as silver nitrate. This research is interested in optimizing the selection of fungi for use in biogenic synthesis of nanoparticles. It is possible that species with the same of minor differences in the niaD sequence may produce similar nanoparticles. A portion of the nitrate reductase gene niaD was amplified from Aspergillus oryzae, A. flavus, A. niger, and A. versicolor obtained from the University of the Philippines Culture Collections. The forward and reverse primers as described by Kitamoto et al (1995) were used, with primers being regarded as conserved for the genus. Of the fungi tested, A. niger did not amplify successfully. The analysis and alignment of the sequences showed large (translated to at least 45 amino acids) differences between any pair of species. Phylogeny analysis using the amplified sequence and homologous sequences of other Aspergillus species showed inconsistencies in distance between the same species. It is therefore recommended that all four species be used in nanoparticle synthesis, as the activity of nitrate reductase can be different in all of these.
format text
author Alba, Laurenzo De Vera
Lagman, Carmen
author_facet Alba, Laurenzo De Vera
Lagman, Carmen
author_sort Alba, Laurenzo De Vera
title Variation of a conserved region in the nitrate reductase gene(NIAD) of selected aspergillus species
title_short Variation of a conserved region in the nitrate reductase gene(NIAD) of selected aspergillus species
title_full Variation of a conserved region in the nitrate reductase gene(NIAD) of selected aspergillus species
title_fullStr Variation of a conserved region in the nitrate reductase gene(NIAD) of selected aspergillus species
title_full_unstemmed Variation of a conserved region in the nitrate reductase gene(NIAD) of selected aspergillus species
title_sort variation of a conserved region in the nitrate reductase gene(niad) of selected aspergillus species
publisher Animo Repository
publishDate 2010
url https://animorepository.dlsu.edu.ph/faculty_research/12052
_version_ 1800918928751329280