Triterpenes from Ardisia squamulosa C. Presl (Myrsinaceae) limit angiogenesis and the expression of von Willebrand factor in duck chorioallantoic membrane

• Main Authors: Raga, Dennis D., Herrera, Annabelle A., Espineli, Dinah L., Shen, Chien Chang, Ragasa, Consolacion Y.
• Format: text
• Published: Animo Repository 2013
• Subjects: Myrsinaceae > Analysis; Von Willebrand factor; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/467
• Institution: De La Salle University

A mixture of bauerenol (1a), a-amyrin (1b) and b-amyrin (1c) in a 3:1:2 ratioobtained from the dichloromethane extract of air-dried leaves of Ardisia squamulosa was tested on duck chorioallantoic membrane (CAM). The mixture of 1a-1c exhibited inhibition of vascularization and formation of ghost vessels on CAMswith 100% embryo survivability at the end of a 9-day treatment period. A possible angio-suppression by inhibition of branch point formation, reduction of inter-capillary distance as well as reduced vascular density in CAMs administered with 1a- 1care also reported. Immunohistochemistry reveals high degree of von Willebrand factor expression and the absence of epithelial membrane antigen. This suggests that the observed angio-suppression was due to the effect of von Willebrand factor in regulation of other angiogenesis modulators.