Triterpenoids and an anti-angiogenic sterol from Ardisia pyramidalis (Cav.) Pers

• Main Authors: Raga, Dennis D., Alimboyoguen, Agnes B., Shen, Chien Chang, Herrera, Annabelle A., Ragasa, Consolacion Y.
• Format: text
• Published: Animo Repository 2011
• Subjects: Myrsinaceae > Analysis; Primulaceae > Analysis; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/476
• Institution: De La Salle University

Chemical constituents of the dichloromethane extract of the leaves of Ardisia pyramidalis (Cav.) Pers were isolated and identified. Chorioallantoic membrane (CAM) vascularity assay was also conducted to determine the probable effects of these chemical constituents on the CAM of mallard duck relative to its potential anti-angiogenic properties. From the dichloromethane extract of the air-dried leaves of A. pyramidalis, we isolated spinasterol 1, spinasteryl acetate 2, a mixture of α-amyrin 3a, β-amyrin 3b and bauerenol 3c at a ratio of 2:1:2, squalene 4, lutein 5 and triglycerides 6. All were identified on the basis of nuclear magnetic resonance (NMR) spectral data. Compounds 1, 6 and the mixture of 3a, 3b and 3c demonstrated a positive vascular damage activity, whereas 1 showed possible anti-angiogenic properties characterized by capillary hemorrhaging and ghost vessels, which eventually led to a non-functional chorioallantoic membrane.