Prevalence of extended spectrum-β- lactamase (MBL)-producing pseudomonas aeruginosa isolates from the Philippine General Hospital

• Main Authors: Balhon, Zulwin R., Cabrera, Esperanza C., Rodriguez, Roslyn D.M.
• Format: text
• Published: Animo Repository 2003
• Subjects: Pseudomonas aeruginosa; Beta lactamases; Anti-infective agents; Philippine General Hospital; Biology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/12986
• Institution: De La Salle University

Pseudomonas aeruginosa isolates from cases of the Burn Unit and Neonatal Intensive Care Unit of the Philippine General Hospital collected from July to September 2003 were studied for their antimicrobial susceptibility profile. All the 24 isolates were found to be resistant to multiple antibiotics belonging to different classes, with 50% of these showing resistances to all of the 11 antimicrobials tested. Resistance to aztreonam was found in 58% of the isolates, while 72% were resistant to ceftazidime; 80% to cefepime; 80% to amikacin; 84% to cefotaxime; 88% to ciprofloxacin; 88% to ceftriaxone; 88% to netilmicin; 92% to gentacimin; 92% to imipenem; and 100% to trimethoprim. Using the double-disc synergy test with clavulanate, aztreonam, ceftazidime, cefepime, and cefotaxime, the isolates were found to be negative for extended-spectrum-β-lactamase (ESBL) production. However, 14 of the 24 isolates or 58% were positive for metallo-β-lactamase (MBL) production using the double-disc test with imipenem and 0.5M EDTA-containing filter paper disc.