Cytotoxic sterols from Philippine mushrooms

• Main Authors: Ragasa, Consolacion Y., Oyong, Glenn G., Tan, Maria Carmen S., de Los Reyes, Mariquit M., de Castro, Maria Ellenita G.
• Format: text
• Published: Animo Repository 2020
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/735; https://animorepository.dlsu.edu.ph/context/faculty_research/article/1734/type/native/viewcontent
• Institution: De La Salle University

© 2020 Chemical Publishing Co.. All rights reserved. Ergosterol peroxide (1) and ergosterol (2) were commonly isolated as the major compounds of Philippine mushrooms. Sterols 1 and 2 from the dichloromethane extract of Geastrum triplex and Termitomyces clypeatus, respectively, were evaluated for their cytotoxic activities against four human cancer cell lines, viz., breast cancer (MCF-7), colon cancer (HT-29), leukemia (THP-1), and small lung cell carcinoma (H69PR), and a human normal cell line, human dermal fibroblast-neonatal (HDFn), using the PrestoBlue® cell viability assay. Compounds 1 and 2 exhibited the strongest activities against HT-29 with IC50 values of 1.79 and 2.98 µg/mL, respectively, while Zeocin gave an IC50 of 4.89 µg/mL. These compounds also exhibited strong antiproliferative effects against MCF-7 with IC50 values of 4.13 for 1 and 4.20 µg/ mL for compound 2, comparable to Zeocin with IC50 = 3.68 µg/mL. Only moderate cytotoxicity resulted when compounds 1 and 2 were tested against H69PR with IC50 values of 7.78 and 6.83 µg/mL, respectively, while Zeocin exhibited an IC50 of 9.81 µg/mL. Furthermore, compounds 1 and 2 showed no effects against THP-1 (IC50 > 100 µg/mL), while Zeocin showed an IC50 of 4.73 µg/mL. Although compounds 1 and 2 have been reported to exhibit different bioactivities in previous studies, the cancer cell lines tested and/or the polarities of the solvents for extraction varied. Therefore, comparisons of the cytotoxic activities of compounds 1 and 2 with earlier studies could not be made extensively.