Extraction, isolation and MALDI-QTOF MS/MS analysis of β-D-Glucan from the fruiting bodies of Daedalea quercina

© 2016 Elsevier B.V. We report for the first time the extraction, isolation, and the proposed structure of a polysaccharide from the fruiting bodies of Daedalea quercina. The monosaccharide composition of D. quercina isolate (DQW1Pa1) was mainly glucose as identified using GC–MS. FTIR-ATR spectrosco...

Full description

Saved in:
Bibliographic Details
Main Authors: Barrientos, Rodell C., Clerigo, Melody M., Paano, Anamy Ma C.
Format: text
Published: Animo Repository 2016
Online Access:https://animorepository.dlsu.edu.ph/faculty_research/738
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: De La Salle University
id oai:animorepository.dlsu.edu.ph:faculty_research-1737
record_format eprints
spelling oai:animorepository.dlsu.edu.ph:faculty_research-17372023-01-23T05:51:31Z Extraction, isolation and MALDI-QTOF MS/MS analysis of β-D-Glucan from the fruiting bodies of Daedalea quercina Barrientos, Rodell C. Clerigo, Melody M. Paano, Anamy Ma C. © 2016 Elsevier B.V. We report for the first time the extraction, isolation, and the proposed structure of a polysaccharide from the fruiting bodies of Daedalea quercina. The monosaccharide composition of D. quercina isolate (DQW1Pa1) was mainly glucose as identified using GC–MS. FTIR-ATR spectroscopy and absolute configuration studies showed that this polysaccharide is a β-D-glucan. Its average molecular weight obtained using size exclusion chromatography was 1.6 × 104 Da, consistent with glucans derived from the order Polyporaceae. MALDI-QTOF MS/MS was carried out to identify the linkage and connectivity of the glucose units. Collision Induced Dissociation (CID) of selected parent ions of different oligosaccharide lengths showed the presence of characteristic glycosidic bond cleavages Bn/Cn, the linear backbone by 1-6 linkage, and the cross-ring fragment, 0,3An. Presence of branching unit was identified from high intensity 0,3A4 fragment and verified from diagnostic ion of [D] and [D-H2O] types. To confirm the linkage assignment obtained using MALDI-QTOF MS/MS, DQW1Pa1 was subjected to methylation analysis. Results showed the presence of 1-3, 1-6, 1- and 1-3-6 linked glucose in the order of decreasing abundance, respectively. The repeating unit of isolate DQW1Pa1 was deduced as 1-3 linked linear glucose backbone with branches composed of three 1-3 linked glucose units connected to backbone by 1-6 linkage. 2016-12-01T08:00:00Z text text/html https://animorepository.dlsu.edu.ph/faculty_research/738 Faculty Research Work Animo Repository
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
description © 2016 Elsevier B.V. We report for the first time the extraction, isolation, and the proposed structure of a polysaccharide from the fruiting bodies of Daedalea quercina. The monosaccharide composition of D. quercina isolate (DQW1Pa1) was mainly glucose as identified using GC–MS. FTIR-ATR spectroscopy and absolute configuration studies showed that this polysaccharide is a β-D-glucan. Its average molecular weight obtained using size exclusion chromatography was 1.6 × 104 Da, consistent with glucans derived from the order Polyporaceae. MALDI-QTOF MS/MS was carried out to identify the linkage and connectivity of the glucose units. Collision Induced Dissociation (CID) of selected parent ions of different oligosaccharide lengths showed the presence of characteristic glycosidic bond cleavages Bn/Cn, the linear backbone by 1-6 linkage, and the cross-ring fragment, 0,3An. Presence of branching unit was identified from high intensity 0,3A4 fragment and verified from diagnostic ion of [D] and [D-H2O] types. To confirm the linkage assignment obtained using MALDI-QTOF MS/MS, DQW1Pa1 was subjected to methylation analysis. Results showed the presence of 1-3, 1-6, 1- and 1-3-6 linked glucose in the order of decreasing abundance, respectively. The repeating unit of isolate DQW1Pa1 was deduced as 1-3 linked linear glucose backbone with branches composed of three 1-3 linked glucose units connected to backbone by 1-6 linkage.
format text
author Barrientos, Rodell C.
Clerigo, Melody M.
Paano, Anamy Ma C.
spellingShingle Barrientos, Rodell C.
Clerigo, Melody M.
Paano, Anamy Ma C.
Extraction, isolation and MALDI-QTOF MS/MS analysis of β-D-Glucan from the fruiting bodies of Daedalea quercina
author_facet Barrientos, Rodell C.
Clerigo, Melody M.
Paano, Anamy Ma C.
author_sort Barrientos, Rodell C.
title Extraction, isolation and MALDI-QTOF MS/MS analysis of β-D-Glucan from the fruiting bodies of Daedalea quercina
title_short Extraction, isolation and MALDI-QTOF MS/MS analysis of β-D-Glucan from the fruiting bodies of Daedalea quercina
title_full Extraction, isolation and MALDI-QTOF MS/MS analysis of β-D-Glucan from the fruiting bodies of Daedalea quercina
title_fullStr Extraction, isolation and MALDI-QTOF MS/MS analysis of β-D-Glucan from the fruiting bodies of Daedalea quercina
title_full_unstemmed Extraction, isolation and MALDI-QTOF MS/MS analysis of β-D-Glucan from the fruiting bodies of Daedalea quercina
title_sort extraction, isolation and maldi-qtof ms/ms analysis of β-d-glucan from the fruiting bodies of daedalea quercina
publisher Animo Repository
publishDate 2016
url https://animorepository.dlsu.edu.ph/faculty_research/738
_version_ 1756432654488567808