Biodiscovery of antibacterial constituents from the endolichenic fungi isolated from Parmotrema rampoddense

• Main Authors: Tan, Mario A., Castro, Sarleen G., Oliva, Patricia Marie P., Yap, Paul Raymund J., Nakayama, Atsushi, Magpantay, Hilbert D., dela Cruz, Thomas Edison E.
• Format: text
• Published: Animo Repository 2020
• Subjects: Lichens; Antibacterial agents; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/1105
• Institution: De La Salle University

A total of nine endolichenic fungi were isolated from the foliose lichen Parmotrema rampoddense (Nyl.) Hale. Of the nine endolichenic fungi, three taxa (Fusarium proliferatum, Nemania primolutea, Daldinia eschsholtzii) showed antibacterial activities as determined by the disk difusion assay against ESKAPE bacterial pathogens. Fusarium proliferatum gave the most active fungal extract with zone of inhibition values of 15 mm and 19 mm against E. faecalis and S. aureus, respectively. Further chromatographic purifcation of the F. proliferatum ethyl acetate extract led to the isolation and identifcation of bis(2-ethylhexyl)terephthalate (1), acetyl tributyl citrate (2), and fusarubin (3). Acetyl tributyl citrate (2) exhibited moderate antibacterial activity against Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus.