Protective influence of Carica papaya L. aqueous leaf extract against hyperuricemia and acute renal injury in a murine model

• Main Authors: Calderon, Pacifico Eric, San Juan, Chrizarah, San Pedro, Mary Grace, Reyes, Ariane Marie, Salom, Patrick Jerome, Sanchez, Arnel Rheuim, Sandigan, Hazel, Sangayab, Heshvey Joy, Saure, Maria Cristine Joy, Savilla, Mar Henrick, Santos, Desmond, Santos, Jamee Lou, Sia, Vincent, Sim, Stephanie, Fontanilla, Fernandino Jose, Ples, Michael
• Format: text
• Published: Animo Repository 2016
• Subjects: Papaya—Therapeutic use; Hyperuricemia—Treatment; Acute renal failure—Treatment; Biology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/1370; https://animorepository.dlsu.edu.ph/context/faculty_research/article/2369/type/native/viewcontent
• Institution: De La Salle University

This study was conducted as a pilot investigation on the protective influence of Carica papaya L. aqueous leaf extract (PLE) against experimental hyperuricemia and acute renal injury in an animal model. The effects of oral pre-treatment with PLE on BUA (blood uric acid) levels and renal histopathology of potassium bromate (KBrO3)-treated adult male albino mice were assessed. Sixty mice were randomized to six groups (n = 10): sham (sterile water) and KBrO3 controls; three experimental groups: PLE1, PLE2 and PLE3 (1, 2 and 3 g/kg body weight [BW] + KBrO3); and ascorbic acid (ascorbic acid 200 mg/kg BW + KBrO3) comparator. Oral pre-treatment with PLE was given for 14 d via gastric gavage. On day 14, except on the sham control, hyperuricemia was induced by oral administration of a single dose of KBrO3 200 mg/kg body weight. BUA levels were measured pre- and post-KBrO3 induction (at 333 h and 336 h, respectively). The kidneys were then immediately excised under anesthesia. Histopathologic evaluation of the kidneys was done using a standard numerical grading scheme. Results showed a significant increase in BUA levels in the KBrO3 control (p < 0.001). In the PLE groups, BUA levels were not significantly increased (p > 0.05). Histologically, there was significant attenuation of acute renal tissue damage in the PLE groups. However, the BUA and histopathologic responses of the PLE groups were not dose-dependent (p = 0.80 and p = 0.66, respectively). These preliminary findings suggest that PLE may have antihyperuricemic and nephroprotective effects in this murine model of hyperuricemia and acute renal tissue injury. © 2016 Author(s).