The genotoxic potential of alugbati leaf extracts on MCF-7 cells

• Main Authors: Garza, Darcy L., Arcilla, Rechel G., Enriquez, Ma Luisa D., Tan, Maria Carmen S., Noel, Marissa G.
• Format: text
• Published: Animo Repository 2019
• Subjects: Basellaceae—Toxicology; Genetic toxicology; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/1610
• Institution: De La Salle University

To determine the genotoxicity of alugbati (Basella alba Linn. var. rubra) leaf extracts on breast adenocarcinoma, the Comet assay was employed on MCF-7 cells incubated with the following: lyophilized alugbati juice extracts reconstituted with 2 % DMSO (AJ) and in aqueous media (AJ2), and lyophilized alugbati hydrolysate (exogenous myrosinase (E.C. 3.2.3.1) assisted) re- dissolved with 2 % DMSO in culture media (AH). Untreated MCF-7 cells in 2 % DMSO served as the negative control. MANOVA and Post hoc Tukey's HSD were employed to determine statistically significant differences among the samples. First, mutant cells in AJ and AH formed pronounced comet tails indicating that DNA damage had occurred significantly compared to that of the control. Post hoc comparisons between AJ and AH indicated that both samples exhibited comparable effects to MCF-7 cells. Due to the similarity of AJ to AH, it was presumed that hydrolysis occurred during the mechanical process of juice extraction. Second, AJ2 exhibited analogous results with the control; whereas, AJ was found to be statistically different. Aberrant cells incubated with the control and AJ2 trials exhibited relatively minimal genotoxicity as evidenced by intact nuclei. Overall, multiple comparisons illustrated that the most prominent DNA damage was observed by extracts AJ and AH in all parameters. The results of this study suggested that alugbati leaves subjected to enzyme-assisted hydrolysis or juice extractions prepared in DMSO caused considerable DNA damage in MCF-7 cells. © 2019 Jordan Journal of Biological Sciences.