High genetic diversity of Anaplasma marginale detected from Philippine cattle

• Main Authors: Ybañez, Adrian Patalinghug, Ybañez, Rochelle Haidee D., Claveria, Florencia G., Flores, Mary Jane Cruz, Xuenan, Xuen, Yokoyama, Naoaki, Inokuma, Hisashi
• Format: text
• Published: Animo Repository 2014
• Subjects: Anaplasma marginale; Cattle—Diseases > Philippines; Biology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/1699; https://animorepository.dlsu.edu.ph/context/faculty_research/article/2698/type/native/viewcontent
• Institution: De La Salle University

A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Msp1a gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and characterization of diverse genotypes of A. marginale in the Philippine cattle. © 2014 The Japanese Society of Veterinary Science.