Cytotoxic cardenolide and sterols from Calotropis gigantea

Cytotoxic cardenolide and sterols from Calotropis gigantea

The dichloromethane extract from the leaves of Calotropis gigantea Linn. was strongly cytotoxic against non-small cell lung carcinoma (A549), colon carcinoma (HCT 116) and hepatocellular carcinoma (Hep G2), and non toxic to Chinese hamster ovary (AA8). The extract afforded uscharin (1), 3,5,8-trihyd...

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Bibliographic Details
Main Authors: Jacinto, Sonia D., Chun, Eunice Alexis C., Montuno, Anthony Sebastian, Shen, Chien Chang, Espineli, Dinah L., Ragasa, Consolacion Y.
Format: text
Published: Animo Repository 2011
Subjects:
Calotropis—Analysis
Asclepiadaceae > Analysis
Cancer
Chemistry
Online Access:https://animorepository.dlsu.edu.ph/faculty_research/1948
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Institution: De La Salle University
Description
Summary:The dichloromethane extract from the leaves of Calotropis gigantea Linn. was strongly cytotoxic against non-small cell lung carcinoma (A549), colon carcinoma (HCT 116) and hepatocellular carcinoma (Hep G2), and non toxic to Chinese hamster ovary (AA8). The extract afforded uscharin (1), 3,5,8-trihydroxy-24-methylcholest-6,22-diene (2), a mixture of (24R)-3β-hydroxy-24-ethylcholest-5-en-7-one (3a) and 6β-hydroxy-24- ethylcholest-4,22-dien-3-one (3b), and another mixture of (24R)-24-ethylcholest- 4-en-3-one (4a) and (24S)-24-ethylcholest-4,22-dien-3-one (4b). Cardenolide 1 exhibited extreme toxicity to A549, HCT 116 and Hep G2 with IC50 values of 0.003 μg/mL, 0.013 μg/mL, and 0.018 μg/mL, respectively, while sample 3 exhibited an IC50 of 1.35 μg/mL, 4.46 μg/mL, and 3.83 ́g/mL, respectively.

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