Chemical constituents and antioxidant potentials of seven Philippine mosses

• Main Authors: Carranza, Mary Stephanie S., Linis, Virgilio C., Ragasa, Consolacion Y., Tan, Maria Carmen S.
• Format: text
• Published: Animo Repository 2019
• Subjects: Bryophytes > Philippines—Analysis; Mosses > Philippines—Analysis; Free radicals (Chemistry); Gas chromatography; Mass spectrometry; X-ray spectroscopy; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/2395
• Institution: De La Salle University

Seven species of Philippine mosses, namely Calyptothecium ramosii Broth., Gymnostomum recurvirostum Hedw., Hypnum plumiforme Wilson, Leucobryum scalare Müll.Hal. ex M.Fleisch., Meteoriopsis reclinata (Müll.Hal.) M.Fleisch., Mitthyridium undulatum (Dozy & Molk.) H.Rob. and Pelekium boniamum (Besch) were examined in this study. The mosses were analyzed using a gas chromatography equipped with a mass spectrometer (GC-EI-MS) and an energy dispersive X-ray fluorescence spectrometer (EDX) to substantiate their possible usage as dietary supplements. EDX results displayed that calcium was the major mineral found in all the moss samples (1.199% to 11.427%). The free radical scavenging activity of the dried dichloromethane moss extracts was highest for G. recurvirostum indicative of the lowest IC50 concentration at 0.236 mg/mL followed by C. ramosii at 0.306 mg/mL and P. boniamum at 0.315 mg/mL. M. undulatum (IC50 = 1.360 mg/mL) and M. reclinata (IC50 = 1.544 mg/mL) had moderate antioxidant activity, whereas, L. scalare (IC50 = 2.120 mg/mL) and H. plumiforme (IC50 = 2.213 mg/mL) had minimal free radical scavenging potential. The possible antioxidant capabilities of each respective bryophyte were correlated to the presence of the following major constituents from GC-EI-MS investigation: in P. boniamum, α-cadinol, (9.11%), caryophyllene (6.39%) and (-)-spathulenol (5.52%); in G. recurvirostum, pentanoic acid, 2,2,4-trimethyl-3-carboxyisopropyl, isobutyl ester (56.26%), caryophyllenyl alcohol (5.96%) and α-cadinene (5.88%); in C. ramosii, phytol (17.86%), and phytol acetate (14.03%). 7,9-di-tert-butyl-1-oxaspiro (4,5) deca-6,9-diene-2,8-dione (9.59%), and 4,8,12,16-tetramethylheptadecan-4-olide (6.11%). Apart from H. plumiforme, all the samples contained considerable concentrations of n-hexadecanoic acid (range from 3.76% to 29.03%). © 2019, Malaysian Society of Analytical Sciences. All rights reserved.