High prevalence of carbapenem and extended spectrum β-lactam resistant Escherichia coli from tilapia (Oreochromis niloticus) from two wet markets in Metro Manila, Philippines

Aim: Multiple drug resistant bacteria are serious health problems worldwide, with carbapenem resistant and extended spectrum-β-lactamase (ESBL) producing Enterobacteriaceae classified by Centers for Disease Control and Prevention under the category of "Urgent Threats" and "Serious Thr...

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Main Authors: Kang, Taeuk, Oyong, Glenn Gallego, Cabrera, Esperanza Chua
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Published: Animo Repository 2020
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/2464
https://animorepository.dlsu.edu.ph/context/faculty_research/article/3463/type/native/viewcontent
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-34632021-09-01T06:49:03Z High prevalence of carbapenem and extended spectrum β-lactam resistant Escherichia coli from tilapia (Oreochromis niloticus) from two wet markets in Metro Manila, Philippines Kang, Taeuk Oyong, Glenn Gallego Cabrera, Esperanza Chua Aim: Multiple drug resistant bacteria are serious health problems worldwide, with carbapenem resistant and extended spectrum-β-lactamase (ESBL) producing Enterobacteriaceae classified by Centers for Disease Control and Prevention under the category of "Urgent Threats" and "Serious Threats", respectively. The study characterized Escherichia coli from Oreochromis niloticus procured from two wet markets in Metro Manila in January and September 2016 for their drug resistance. Methodology and results: Antimicrobial susceptibility profiles were determined using standard disc diffusion method. Extended-spectrum β-lactamase production was confirmed using clavulanate double disc synergy assay, carbapenemase production was tested using modified Hodge test, and MBL (metallo-β-lactamase) production was tested using EDTA double disc synergy assay. Results show that of the 25 isolated E. coli, 24 or 96% were resistant to at least one antimicrobial, with 60% being multiple drug resistant. These strains exhibited 20 different resistance phenotypes, suggesting these were different strains. Fifteen of the isolates (60%) screened positive for ESBL. Among these, 11 lost their resistance, indicating the instability of the resistance genes in the host, a characteristic of plasmid-mediated ESBL production. The ESBL suspects tested were confirmed to be ESBL producers. A high 48% of isolates were found to be resistant to carbapenems, with eight of the 11 tested (73%) being positive for carbapenemase production. MBL positive isolates carried the blaIMP gene as determined by multiplex PCR and nucleotide sequencing. Conclusion, significance and impact of study: Study showed a high prevalence of multiple drug resistant E. coli isolates from the commonly-consumed Tilapia procured from the wet markets. This result is compounded by the alarmingly high prevalence of carbapenem resistant and ESBL-producing strains among these isolates. Considering that the genes coding for these resistances are found in mobile genetic elements such as plasmids and integrons that can be transferred to other bacteria resulting to a rapid increase in drug resistant strains, it is highly imperative for all the concerned government units to establish a well-coordinated national surveillance program to monitor and address the occurrence and increase in drug resistant microorganisms in man, animals and the environment. In addition, prudent use of antimicrobials among these should be seriously instituted. © 2020 Universiti Sains Malaysia. 2020-01-01T08:00:00Z text text/html https://animorepository.dlsu.edu.ph/faculty_research/2464 https://animorepository.dlsu.edu.ph/context/faculty_research/article/3463/type/native/viewcontent Faculty Research Work Animo Repository Escherichia coli infections in animals--Philippines--Metro Manila Nile tilapia Drug resistance in microorganisms Enterobacteriaceae Beta lactamases Biology
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Escherichia coli infections in animals--Philippines--Metro Manila
Nile tilapia
Drug resistance in microorganisms
Enterobacteriaceae
Beta lactamases
Biology
spellingShingle Escherichia coli infections in animals--Philippines--Metro Manila
Nile tilapia
Drug resistance in microorganisms
Enterobacteriaceae
Beta lactamases
Biology
Kang, Taeuk
Oyong, Glenn Gallego
Cabrera, Esperanza Chua
High prevalence of carbapenem and extended spectrum β-lactam resistant Escherichia coli from tilapia (Oreochromis niloticus) from two wet markets in Metro Manila, Philippines
description Aim: Multiple drug resistant bacteria are serious health problems worldwide, with carbapenem resistant and extended spectrum-β-lactamase (ESBL) producing Enterobacteriaceae classified by Centers for Disease Control and Prevention under the category of "Urgent Threats" and "Serious Threats", respectively. The study characterized Escherichia coli from Oreochromis niloticus procured from two wet markets in Metro Manila in January and September 2016 for their drug resistance. Methodology and results: Antimicrobial susceptibility profiles were determined using standard disc diffusion method. Extended-spectrum β-lactamase production was confirmed using clavulanate double disc synergy assay, carbapenemase production was tested using modified Hodge test, and MBL (metallo-β-lactamase) production was tested using EDTA double disc synergy assay. Results show that of the 25 isolated E. coli, 24 or 96% were resistant to at least one antimicrobial, with 60% being multiple drug resistant. These strains exhibited 20 different resistance phenotypes, suggesting these were different strains. Fifteen of the isolates (60%) screened positive for ESBL. Among these, 11 lost their resistance, indicating the instability of the resistance genes in the host, a characteristic of plasmid-mediated ESBL production. The ESBL suspects tested were confirmed to be ESBL producers. A high 48% of isolates were found to be resistant to carbapenems, with eight of the 11 tested (73%) being positive for carbapenemase production. MBL positive isolates carried the blaIMP gene as determined by multiplex PCR and nucleotide sequencing. Conclusion, significance and impact of study: Study showed a high prevalence of multiple drug resistant E. coli isolates from the commonly-consumed Tilapia procured from the wet markets. This result is compounded by the alarmingly high prevalence of carbapenem resistant and ESBL-producing strains among these isolates. Considering that the genes coding for these resistances are found in mobile genetic elements such as plasmids and integrons that can be transferred to other bacteria resulting to a rapid increase in drug resistant strains, it is highly imperative for all the concerned government units to establish a well-coordinated national surveillance program to monitor and address the occurrence and increase in drug resistant microorganisms in man, animals and the environment. In addition, prudent use of antimicrobials among these should be seriously instituted. © 2020 Universiti Sains Malaysia.
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author Kang, Taeuk
Oyong, Glenn Gallego
Cabrera, Esperanza Chua
author_facet Kang, Taeuk
Oyong, Glenn Gallego
Cabrera, Esperanza Chua
author_sort Kang, Taeuk
title High prevalence of carbapenem and extended spectrum β-lactam resistant Escherichia coli from tilapia (Oreochromis niloticus) from two wet markets in Metro Manila, Philippines
title_short High prevalence of carbapenem and extended spectrum β-lactam resistant Escherichia coli from tilapia (Oreochromis niloticus) from two wet markets in Metro Manila, Philippines
title_full High prevalence of carbapenem and extended spectrum β-lactam resistant Escherichia coli from tilapia (Oreochromis niloticus) from two wet markets in Metro Manila, Philippines
title_fullStr High prevalence of carbapenem and extended spectrum β-lactam resistant Escherichia coli from tilapia (Oreochromis niloticus) from two wet markets in Metro Manila, Philippines
title_full_unstemmed High prevalence of carbapenem and extended spectrum β-lactam resistant Escherichia coli from tilapia (Oreochromis niloticus) from two wet markets in Metro Manila, Philippines
title_sort high prevalence of carbapenem and extended spectrum β-lactam resistant escherichia coli from tilapia (oreochromis niloticus) from two wet markets in metro manila, philippines
publisher Animo Repository
publishDate 2020
url https://animorepository.dlsu.edu.ph/faculty_research/2464
https://animorepository.dlsu.edu.ph/context/faculty_research/article/3463/type/native/viewcontent
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