Teratogenic effect of papaya (Carica papaya) seed extracts on the embryonic development of zebrafish (Danio rerio)

• Main Authors: De Castro, Maria Ellenita G., Dulay, Rich Milton R., De La Salle University, Manila
• Format: text
• Published: Animo Repository 2015
• Subjects: Papaya—Seeds; Teratogenicity testing; Zebra danio—Embryos
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/2519
• Institution: De La Salle University

With the teratogenic assay using D. rerio embryonic model, this present work established the toxic and teratogenic effects of extracts of the two C. papaya seed types, young and mature. Bioactive compositions were obtained through hot water extraction. Embryos at segmentation phase were exposed to the varying extract concentrations. Mortality, hatchability, and morphological malformations were determined. Based on the results, the incidences of mortality and most teratogenic effects were apparently concentration-dependent. Embryos exposed at 10% of young seed extract and 3% of mature seed extract significantly recorded 100% mortality after 48 hours of treatment exposure. Coagulation of embryo was the most notable toxic effect of both extracts. Hatchability of 0.1% and 0.3% treated embryos was significantly lower than that of the control embryos. No hatched was recorded in 1% or higher concentrations. Embryos at 0.1% (young seed) and 0.03% (mature seed) or higher extract concentrations showed delayed development. Tail malformation (bent tail or hook-like tail) is a fingerprint morphological endpoint of both C. papaya seed extracts. At 72 hour post treatment application (hpta), tail malformation was observed in embryos at 0.03% (for mature seed) and 0.1% (for young seed) or higher concentrations. Altogether, the extracts of the two C. papaya seed types affect the survival and embryonic development of D. rerio, however, mature seed extract is found more toxic than the young seed extract. © 2015 AENSI Publisher All rights reserved.