Immunohistochemical detection of S-100 in the spleen of Philippine domestic cat

• Main Authors: Vitor, Rodel Jonathan S., Ostwani, Khaled
• Format: text
• Published: Animo Repository 2018
• Subjects: Immunohistochemistry; Cats; Spleen; Biology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/2534
• Institution: De La Salle University

The spleens from 20 (ten male and ten female) two to five year-old apparently healthy domestic cats (Felis catus L.) were collected and examined by immunohistochemical methods to identify S-100 immunoreactive structures. In all sections of the spleen, S-100 was found in the nerve elements, endothelial cells lining the capsular arteries, trabecular arteries, penicillar arterioles and sheathed capillaries in the red pulp, splenic arterioles in the white pulp, as well as in the macrophages and reticular cells that formed a sleeve around the penicillar arterioles and sheathed capillaries of the spleen, lymphoid follicles and periarteriolar lymphatic sheaths (PALS) of the white pulp. On the other hand, S-100 was not observed in the veins in both the stroma and parenchyma. It is hypothesized that S-100 in the endothelial cells of sheathed capillaries is suggestive of its function in regulating the semi-permeability of blood vessels, while the demonstration of S-100 in the nerves may indicate that it is transported from gliocytes, secreting it to the target cells. The present findings showed that S-100 is positive in nerve elements, endothelial linings of arterial vessels and capillaries, lymphoid follicles and PALSof the Philippine domestic cat. © 2018 University of the Philippines at Los Banos. All Rights Reserved.