Determining the apoptotic-inducing property of isothiocyanates extracted from three cultivars of Raphanus sativus Linn. using the comet assay

The apoptotic inducing property of three cultivars of Raphanus sativus L.: white or daikon, red or cherry belle, and large red, were tested on three cancer cell lines: MCF-7, K562, and HT-29. The wild type PAE cells were also incubated with radish extracts for genotoxicity. Each cell line was cultur...

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Main Authors: Tan, Maria Carmen S., Enriquez, Ma. Luisa D., Arcilla, Rechel G., Noel, Marissa G.
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Published: Animo Repository 2017
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/2824
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-38232021-11-09T06:54:27Z Determining the apoptotic-inducing property of isothiocyanates extracted from three cultivars of Raphanus sativus Linn. using the comet assay Tan, Maria Carmen S. Enriquez, Ma. Luisa D. Arcilla, Rechel G. Noel, Marissa G. The apoptotic inducing property of three cultivars of Raphanus sativus L.: white or daikon, red or cherry belle, and large red, were tested on three cancer cell lines: MCF-7, K562, and HT-29. The wild type PAE cells were also incubated with radish extracts for genotoxicity. Each cell line was cultured and treated with either one kind of juice extract or enzymatically hydrolyzed radish sample for 24 hours before harvesting and processing for single cell gel electrophoresis. For each culture set-up, 50 images of cells were captured and analyzed for DNA damage based on three parameters: tail length (TL), percent DNA in the tail (D), and tail moment (TM). Multiple comparisons using Tukey's HSD procedure showed that juice extracts resulted to substantial genotoxicity in MCF-7 cells among the cancer lines based on the superior TL, D, and TM of the comets having 95% CI of [161.16, 231.44], [33.85, 46.63], and [99.97, 157.91], respectively. The juice extracts exhibited no genotoxicity towards normal PAE cells. Extracts from a β-thioglucosidase assisted hydrolysis of white tubers manifested significant disruption of DNA integrity of MCF-7 cells than that of the red tubers (p < 0.0001). Furthermore, DNA fragmentation for MCF-7 cells treated with juice and semi-purified preparations of the white tuber were found to be similar with 95% CI for mean differences of [-112.81, 24.21] for TL, [-18.03, 5.93] for D and [-56.72, 52.15] for TM. From the results, it can be construed that preparations of R. sativus L. can be a substantial source of biologically active constituents that have the ability to cleave DNA in mutant cell lines while being non-genotoxic to wild type immortalized cells. © 2017 Maria Carmen S. Tan et al. 2017-09-01T07:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/2824 Faculty Research Work Animo Repository Radishes--Analysis Glucosinolates Cell lines Genetic toxicology Chemistry
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Radishes--Analysis
Glucosinolates
Cell lines
Genetic toxicology
Chemistry
spellingShingle Radishes--Analysis
Glucosinolates
Cell lines
Genetic toxicology
Chemistry
Tan, Maria Carmen S.
Enriquez, Ma. Luisa D.
Arcilla, Rechel G.
Noel, Marissa G.
Determining the apoptotic-inducing property of isothiocyanates extracted from three cultivars of Raphanus sativus Linn. using the comet assay
description The apoptotic inducing property of three cultivars of Raphanus sativus L.: white or daikon, red or cherry belle, and large red, were tested on three cancer cell lines: MCF-7, K562, and HT-29. The wild type PAE cells were also incubated with radish extracts for genotoxicity. Each cell line was cultured and treated with either one kind of juice extract or enzymatically hydrolyzed radish sample for 24 hours before harvesting and processing for single cell gel electrophoresis. For each culture set-up, 50 images of cells were captured and analyzed for DNA damage based on three parameters: tail length (TL), percent DNA in the tail (D), and tail moment (TM). Multiple comparisons using Tukey's HSD procedure showed that juice extracts resulted to substantial genotoxicity in MCF-7 cells among the cancer lines based on the superior TL, D, and TM of the comets having 95% CI of [161.16, 231.44], [33.85, 46.63], and [99.97, 157.91], respectively. The juice extracts exhibited no genotoxicity towards normal PAE cells. Extracts from a β-thioglucosidase assisted hydrolysis of white tubers manifested significant disruption of DNA integrity of MCF-7 cells than that of the red tubers (p < 0.0001). Furthermore, DNA fragmentation for MCF-7 cells treated with juice and semi-purified preparations of the white tuber were found to be similar with 95% CI for mean differences of [-112.81, 24.21] for TL, [-18.03, 5.93] for D and [-56.72, 52.15] for TM. From the results, it can be construed that preparations of R. sativus L. can be a substantial source of biologically active constituents that have the ability to cleave DNA in mutant cell lines while being non-genotoxic to wild type immortalized cells. © 2017 Maria Carmen S. Tan et al.
format text
author Tan, Maria Carmen S.
Enriquez, Ma. Luisa D.
Arcilla, Rechel G.
Noel, Marissa G.
author_facet Tan, Maria Carmen S.
Enriquez, Ma. Luisa D.
Arcilla, Rechel G.
Noel, Marissa G.
author_sort Tan, Maria Carmen S.
title Determining the apoptotic-inducing property of isothiocyanates extracted from three cultivars of Raphanus sativus Linn. using the comet assay
title_short Determining the apoptotic-inducing property of isothiocyanates extracted from three cultivars of Raphanus sativus Linn. using the comet assay
title_full Determining the apoptotic-inducing property of isothiocyanates extracted from three cultivars of Raphanus sativus Linn. using the comet assay
title_fullStr Determining the apoptotic-inducing property of isothiocyanates extracted from three cultivars of Raphanus sativus Linn. using the comet assay
title_full_unstemmed Determining the apoptotic-inducing property of isothiocyanates extracted from three cultivars of Raphanus sativus Linn. using the comet assay
title_sort determining the apoptotic-inducing property of isothiocyanates extracted from three cultivars of raphanus sativus linn. using the comet assay
publisher Animo Repository
publishDate 2017
url https://animorepository.dlsu.edu.ph/faculty_research/2824
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