Evaluation of 16S rRNA gene PCR and culture of buffy coat, 16srRNA gene PCR of whole blood in comparison with whole blood culture for detection of bacteremia

• Main Authors: Valle, Demetrio L., Jr., Andrade, Jeannie I., Cabrera, Esperanza C., Rivera, Windell L.
• Format: text
• Published: Animo Repository 2022
• Subjects: Bacteremia; Blood—Buffy coat; Leucocytes; Polymerase chain reaction; Pathogenic bacteria; Biology; Pathological Conditions, Signs and Symptoms
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/5088
• Institution: De La Salle University

The use of Gram-type specific PCR on buffy coat from clinical specimens for the detection of bacteremia was evaluated for the first time using whole blood culture as the gold standard. In addition, the established buffy coat culture and whole blood PCR were also compared. Gram positive bacteria belo...