Structure elucidation of β-stigmasterol and β-sitosterol from Sesbania grandiflora (Linn.) pers. and β-carotene from Heliotropium indicum Linn. by NMR spectroscopy

• Main Authors: Cayme, Jan-Michael C., Ragasa, Consolacion Y.
• Format: text
• Published: Animo Repository 2004
• Subjects: Sesbania—Analysis; Heliotropium—Analysis; Anti-infective agents; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/5726
• Institution: De La Salle University

The flowers of Sesbania grandiflora (Linn.) Pers. afforded a 3:2 mixture of β-stigmasterol (1a) and β-sitosterol (1b), and oleanolic acid (2), while the leaves of Heliotropium indicum Linn. yielded β-carotene (3), lutein ( 4), a mixture of polyprenols (5), and a mixture of β-stigmasterol and β-sitosterol. The structures of 1a, 1b, and 3 were elucidated by extensive 1D and 2D NMR analyses. Antimicrobial activity tests on 3 indicated that it has high activity against Pseudomonas aeruginosa and Aspergillus niger, slight activity against Staphylococcus aureus, Escherichia coli, Candida albicans, and Trichophyton mentagrophytes, and inactive against Bacillus subtilis.