Studies on the mutagenicity, clastogenicity and antimutagenicity potential of Tinospora rumphii Boerlage (family Menispermaceae)

Extractives prepared from the stems of Tinospora rumphil Boerlage were tested for mutagenic, clastogenic and antimutagenic potential by conducting both in vitro and in vivo tests. The in vitro Rec Assay method, using mutant strains of Bacillus subtilis H17 Rec+ and M45 Rec- was used to test for the...

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Main Author: del Fierro, Ramon S.
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Published: Animo Repository 1983
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/6223
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-70182022-06-27T05:51:37Z Studies on the mutagenicity, clastogenicity and antimutagenicity potential of Tinospora rumphii Boerlage (family Menispermaceae) del Fierro, Ramon S. Extractives prepared from the stems of Tinospora rumphil Boerlage were tested for mutagenic, clastogenic and antimutagenic potential by conducting both in vitro and in vivo tests. The in vitro Rec Assay method, using mutant strains of Bacillus subtilis H17 Rec+ and M45 Rec- was used to test for the DNA-damaging effect of T. rumphii. The results showed that the extractives did not exhibit any DNA-damaging capacity. The Pour Plate method of Ames, another in vitro test, using mutant strains of Salmonella typhimurium TA 1535, TA 1537, TA 97 and TA 98, was used to evaluate the mutagenicity before metabolic activation of the extractives from T. rumphii. The result showed that the extractives do not contain direct mutagens. The Host-Mediated Assay, an in vivo test, was conducted using albino mice (Japanese Namru strain) with S. typhimurium strain His G46 as the indicator organism. The result showed that the extractives from T. rumphii were not transformed into mutagenic product(s) after metabolic activation. The micronucleus Test, using albino mice (Japanese Namru strain) as the test system, showed that the extractives from T. rumphii generally do not affect the chromatin material of the bone marrow cells. However, at a concentration much higher than the recommended formula of a decoction which is 20% (w/v), a slight chromosomal damage was observed. The antimutagenic effect on bone marrow cells was evaluated using the Micronucleus Test of Schmid. The mutacarcinogens used were dimethyInitrosamine, flagyl, mitomycin C and safrole. Reduction of micronucleated polychromatic erythrocytes was observed when the extractives from T. rumphii were given orally after administration of the mutacarcinogens. This indicates the antimutagenic property of T. rumphii. 1983-01-01T08:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/6223 Faculty Research Work Animo Repository Menispermaceae—Analysis Chemistry
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Menispermaceae—Analysis
Chemistry
spellingShingle Menispermaceae—Analysis
Chemistry
del Fierro, Ramon S.
Studies on the mutagenicity, clastogenicity and antimutagenicity potential of Tinospora rumphii Boerlage (family Menispermaceae)
description Extractives prepared from the stems of Tinospora rumphil Boerlage were tested for mutagenic, clastogenic and antimutagenic potential by conducting both in vitro and in vivo tests. The in vitro Rec Assay method, using mutant strains of Bacillus subtilis H17 Rec+ and M45 Rec- was used to test for the DNA-damaging effect of T. rumphii. The results showed that the extractives did not exhibit any DNA-damaging capacity. The Pour Plate method of Ames, another in vitro test, using mutant strains of Salmonella typhimurium TA 1535, TA 1537, TA 97 and TA 98, was used to evaluate the mutagenicity before metabolic activation of the extractives from T. rumphii. The result showed that the extractives do not contain direct mutagens. The Host-Mediated Assay, an in vivo test, was conducted using albino mice (Japanese Namru strain) with S. typhimurium strain His G46 as the indicator organism. The result showed that the extractives from T. rumphii were not transformed into mutagenic product(s) after metabolic activation. The micronucleus Test, using albino mice (Japanese Namru strain) as the test system, showed that the extractives from T. rumphii generally do not affect the chromatin material of the bone marrow cells. However, at a concentration much higher than the recommended formula of a decoction which is 20% (w/v), a slight chromosomal damage was observed. The antimutagenic effect on bone marrow cells was evaluated using the Micronucleus Test of Schmid. The mutacarcinogens used were dimethyInitrosamine, flagyl, mitomycin C and safrole. Reduction of micronucleated polychromatic erythrocytes was observed when the extractives from T. rumphii were given orally after administration of the mutacarcinogens. This indicates the antimutagenic property of T. rumphii.
format text
author del Fierro, Ramon S.
author_facet del Fierro, Ramon S.
author_sort del Fierro, Ramon S.
title Studies on the mutagenicity, clastogenicity and antimutagenicity potential of Tinospora rumphii Boerlage (family Menispermaceae)
title_short Studies on the mutagenicity, clastogenicity and antimutagenicity potential of Tinospora rumphii Boerlage (family Menispermaceae)
title_full Studies on the mutagenicity, clastogenicity and antimutagenicity potential of Tinospora rumphii Boerlage (family Menispermaceae)
title_fullStr Studies on the mutagenicity, clastogenicity and antimutagenicity potential of Tinospora rumphii Boerlage (family Menispermaceae)
title_full_unstemmed Studies on the mutagenicity, clastogenicity and antimutagenicity potential of Tinospora rumphii Boerlage (family Menispermaceae)
title_sort studies on the mutagenicity, clastogenicity and antimutagenicity potential of tinospora rumphii boerlage (family menispermaceae)
publisher Animo Repository
publishDate 1983
url https://animorepository.dlsu.edu.ph/faculty_research/6223
_version_ 1767196488208744448