Phenotypic characterization of pink pigmented facultative methylothrophic (PPFM) bacteria from various sources

• Main Authors: Carvajal, Thaddeus M., Dolleton, M.A. M., Gagui, O. A., Peralta, E. C., Valera, M.C. D., Lee, Anthony C.
• Format: text
• Published: Animo Repository 2007
• Subjects: Methylotrophic bacteria; Biology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/7774
• Institution: De La Salle University

A total of eighteen (18) pink-pigmented facultative methylotrophic (PPFM) bacteria from various habitats were isolated and described. A mineral salt agar medium with 0.5% methanol was used as the selective medium for the isolation. Pure cultures were obtained after re-streaking in selective as well as non-selective media. The obtained isolated were gram-negative rods with some unstained cyst like structures. The cyst like structures stained black after polyß-hydroxybutyrate staining with Sudan Black. All of the isolates grew in brain heart infusion agar, nutrient agar, glycerol peptone agar, and eosin methylene blue agar but not in MacConkey agar. Optimum incubation temperature for the isolates varied from room temperature (250C) and 370C. The isolates exhibited varied biological activities based on the different biochemical tests performed.