DNA markers for capsicum species found in the Philippines

• Main Authors: Llanos, Nancy Lazaro, Alcera, R. K., Cruz, A. K., Iriarte, K., Tan, Raymond Girard R., Villanueva, P. M.
• Format: text
• Published: Animo Repository 2002
• Subjects: Peppers—Philippines—Classification—Molecular aspects; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/7758
• Institution: De La Salle University

Molecular markers were established for five Capsicum species found in the Philippines. The isolation of DNA from the leaves of varieties of Capsicum annum and Capsicum frustescens was optimized using the CTAB method. The concentration and purity of the DNA were determined by evaluating the relative absorbance at λ260/280 by UV-V is spectroscopy. The isolated DNA amples were digested by restriction enzymes EcoRl, HindIII and EcoRl/HindIII mixture, and the resulting fragments were analyzed by agarose gel electrophoresis and pulse-field gel electrophoresis (PFGE). The bands were stained with ethidium bromide, viewed under ultraviolet light, and recorded with a digital camera. With the DigiGenius SynGene Tools Software, the total DNA and Restriction Fragment Length Polymorphism (RFLPs) were analyzed. The molecular weight of the total DNA from Capsicum annum, Capsicum frutescens, Capsicum frutescens L, and Capsicum frutescens var. and Capsicum annum var. longum were 527.95, 507.41, 323.65, 363.02 and 277.21 kilobase pairs (kbp), respectively. The variation in fragmentation patterns of Capsicum annuum and Capsicum frutescens was principally due to the difference in size and base composition of their DNA. The RFLP·s generated can serve as a fingerprint to distinguish Capsicum species. Molecular markers are important tools in taxonomic classification. Results from this research will also be valuable in the fields of biotechnology, genetic engineering and food technology.