Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials

The growing international demand for herbal-based products has prompted the need for more stringent quality control methods to detect substandard and adulterated herbal plant materials. However, most prescreening methods, such as thin-layer chromatography (TLC), remain inaccessible to herbal produce...

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Main Author: Enriquez, Erwin
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Published: Archīum Ateneo 2019
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Online Access:https://archium.ateneo.edu/chemistry-faculty-pubs/2
https://pubs.rsc.org/en/content/articlelanding/2019/AY/C9AY01714C#!divAbstract
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Institution: Ateneo De Manila University
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spelling ph-ateneo-arc.chemistry-faculty-pubs-10012020-01-23T02:48:52Z Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials Enriquez, Erwin The growing international demand for herbal-based products has prompted the need for more stringent quality control methods to detect substandard and adulterated herbal plant materials. However, most prescreening methods, such as thin-layer chromatography (TLC), remain inaccessible to herbal producers in resource-limited settings. Here, we report a proof-of-concept demonstration of a multi-stationary phase thin-layer chromatography (MSP-TLC) method for the preliminary on-site identification and evaluation of herbal materials. Our method is based on a unique TLC plate design that features multiple phenyl- and octyl-modified silica gel stationary phases configured as radial sectors. The modified stationary phase patterns were fabricated by screen-printing organosilane solutions onto commercial silica gel TLC plates. Radial elution with an ethanol–water mobile phase from the center of the MSP-TLC plate generates multiple chromatographic profiles simultaneously for a single sample extract. To facilitate the interpretation of the multiple TLC profiles, the MSP-TLC system was coupled with image analysis and chemometric pattern recognition to classify a sample as “within-specifications” or “off-specifications” for a given herbal plant species. Application of the system to Blumea balsamifera and Vitex negundo demonstrated sensitivity and specificity rates that range from 73.1 to 95.1% compared to the respective standard Pharmacopeia TLC methods. The presented method holds considerable promise as a cost-effective, user-friendly on-site prescreening tool for herbal materials in resource-limited settings. 2019-01-01T08:00:00Z text https://archium.ateneo.edu/chemistry-faculty-pubs/2 https://pubs.rsc.org/en/content/articlelanding/2019/AY/C9AY01714C#!divAbstract Chemistry Faculty Publications Archīum Ateneo Analytical Chemistry Biochemistry Chemistry
institution Ateneo De Manila University
building Ateneo De Manila University Library
country Philippines
collection archium.Ateneo Institutional Repository
topic Analytical Chemistry
Biochemistry
Chemistry
spellingShingle Analytical Chemistry
Biochemistry
Chemistry
Enriquez, Erwin
Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials
description The growing international demand for herbal-based products has prompted the need for more stringent quality control methods to detect substandard and adulterated herbal plant materials. However, most prescreening methods, such as thin-layer chromatography (TLC), remain inaccessible to herbal producers in resource-limited settings. Here, we report a proof-of-concept demonstration of a multi-stationary phase thin-layer chromatography (MSP-TLC) method for the preliminary on-site identification and evaluation of herbal materials. Our method is based on a unique TLC plate design that features multiple phenyl- and octyl-modified silica gel stationary phases configured as radial sectors. The modified stationary phase patterns were fabricated by screen-printing organosilane solutions onto commercial silica gel TLC plates. Radial elution with an ethanol–water mobile phase from the center of the MSP-TLC plate generates multiple chromatographic profiles simultaneously for a single sample extract. To facilitate the interpretation of the multiple TLC profiles, the MSP-TLC system was coupled with image analysis and chemometric pattern recognition to classify a sample as “within-specifications” or “off-specifications” for a given herbal plant species. Application of the system to Blumea balsamifera and Vitex negundo demonstrated sensitivity and specificity rates that range from 73.1 to 95.1% compared to the respective standard Pharmacopeia TLC methods. The presented method holds considerable promise as a cost-effective, user-friendly on-site prescreening tool for herbal materials in resource-limited settings.
format text
author Enriquez, Erwin
author_facet Enriquez, Erwin
author_sort Enriquez, Erwin
title Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials
title_short Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials
title_full Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials
title_fullStr Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials
title_full_unstemmed Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials
title_sort radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials
publisher Archīum Ateneo
publishDate 2019
url https://archium.ateneo.edu/chemistry-faculty-pubs/2
https://pubs.rsc.org/en/content/articlelanding/2019/AY/C9AY01714C#!divAbstract
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