13C Nuclear Magnetic Resonance Phytochemical Profiling of the In Vitro Antidiabetic Potential of Vitex negundo L.

Vitex negundo has been known since ancient times as a medicinal plant. The objective of this study is to investigate the effect of methanol and ethanol extracts, and ethyl acetate, chloroform and aqueous fractions of Vitex negundo using an in vitro model to test glucose diffusion and to determine th...

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Main Authors: Zabala, Myrnille Joy B, Lagurin, Lolita G, Dayrit, Fabian M
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Published: Archīum Ateneo 2016
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Online Access:https://archium.ateneo.edu/chemistry-faculty-pubs/130
https://jurnal.unai.edu/index.php/isc/article/view/1652
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spelling ph-ateneo-arc.chemistry-faculty-pubs-11292020-07-28T08:39:32Z 13C Nuclear Magnetic Resonance Phytochemical Profiling of the In Vitro Antidiabetic Potential of Vitex negundo L. Zabala, Myrnille Joy B Lagurin, Lolita G Dayrit, Fabian M Vitex negundo has been known since ancient times as a medicinal plant. The objective of this study is to investigate the effect of methanol and ethanol extracts, and ethyl acetate, chloroform and aqueous fractions of Vitex negundo using an in vitro model to test glucose diffusion and to determine the phytochemical profile of the extracts and fractions using 13C nuclear magnetic resonance (NMR) spectroscopy. The chloroform fractions, ethyl acetate-EtOH and ethyl acetate-MeOH gave the highest inhibitory effect on both the diffusion activities in vitro. Retardation of glucose diffusion suggests that negundo has the potential to lower postprandial glucose. Correlation analysis of the 13C NMR profile with retardation activity suggests that compounds containing glycosidic residues may be responsible for the glucose retardation activity. This is the first example where activity has been correlated with specific structural features of compounds from a crude extract using 13C NMR chemical shifts to assist in the identification of active compounds. 2016-01-01T08:00:00Z text https://archium.ateneo.edu/chemistry-faculty-pubs/130 https://jurnal.unai.edu/index.php/isc/article/view/1652 Chemistry Faculty Publications Archīum Ateneo Vitex negundo in vitro glucose diffusion retardation index antidiabetic 13C NMR Chemistry Medicinal and Pharmaceutical Chemistry
institution Ateneo De Manila University
building Ateneo De Manila University Library
country Philippines
collection archium.Ateneo Institutional Repository
topic Vitex negundo
in vitro
glucose diffusion retardation index
antidiabetic
13C NMR
Chemistry
Medicinal and Pharmaceutical Chemistry
spellingShingle Vitex negundo
in vitro
glucose diffusion retardation index
antidiabetic
13C NMR
Chemistry
Medicinal and Pharmaceutical Chemistry
Zabala, Myrnille Joy B
Lagurin, Lolita G
Dayrit, Fabian M
13C Nuclear Magnetic Resonance Phytochemical Profiling of the In Vitro Antidiabetic Potential of Vitex negundo L.
description Vitex negundo has been known since ancient times as a medicinal plant. The objective of this study is to investigate the effect of methanol and ethanol extracts, and ethyl acetate, chloroform and aqueous fractions of Vitex negundo using an in vitro model to test glucose diffusion and to determine the phytochemical profile of the extracts and fractions using 13C nuclear magnetic resonance (NMR) spectroscopy. The chloroform fractions, ethyl acetate-EtOH and ethyl acetate-MeOH gave the highest inhibitory effect on both the diffusion activities in vitro. Retardation of glucose diffusion suggests that negundo has the potential to lower postprandial glucose. Correlation analysis of the 13C NMR profile with retardation activity suggests that compounds containing glycosidic residues may be responsible for the glucose retardation activity. This is the first example where activity has been correlated with specific structural features of compounds from a crude extract using 13C NMR chemical shifts to assist in the identification of active compounds.
format text
author Zabala, Myrnille Joy B
Lagurin, Lolita G
Dayrit, Fabian M
author_facet Zabala, Myrnille Joy B
Lagurin, Lolita G
Dayrit, Fabian M
author_sort Zabala, Myrnille Joy B
title 13C Nuclear Magnetic Resonance Phytochemical Profiling of the In Vitro Antidiabetic Potential of Vitex negundo L.
title_short 13C Nuclear Magnetic Resonance Phytochemical Profiling of the In Vitro Antidiabetic Potential of Vitex negundo L.
title_full 13C Nuclear Magnetic Resonance Phytochemical Profiling of the In Vitro Antidiabetic Potential of Vitex negundo L.
title_fullStr 13C Nuclear Magnetic Resonance Phytochemical Profiling of the In Vitro Antidiabetic Potential of Vitex negundo L.
title_full_unstemmed 13C Nuclear Magnetic Resonance Phytochemical Profiling of the In Vitro Antidiabetic Potential of Vitex negundo L.
title_sort 13c nuclear magnetic resonance phytochemical profiling of the in vitro antidiabetic potential of vitex negundo l.
publisher Archīum Ateneo
publishDate 2016
url https://archium.ateneo.edu/chemistry-faculty-pubs/130
https://jurnal.unai.edu/index.php/isc/article/view/1652
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