Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications

As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future outbreaks. The current gold standard for molecular diagnostics is...

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Main Authors: Moore, Keith J M, Mason, Christopher E, co-authors, 56
Format: text
Published: Archīum Ateneo 2021
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Online Access:https://archium.ateneo.edu/chemistry-faculty-pubs/185
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802757/
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spelling ph-ateneo-arc.chemistry-faculty-pubs-11852022-05-12T06:20:49Z Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications Moore, Keith J M Mason, Christopher E co-authors, 56 As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future outbreaks. The current gold standard for molecular diagnostics is the polymerase chain reaction (PCR), but the extraordinary and unmet demand for testing in a variety of environments means that both complementary and supplementary testing solutions are still needed. This review highlights the role that loop-mediated isothermal amplification (LAMP) has had in filling this global testing need, providing a faster and easier means of testing, and what it can do for future applications, pathogens, and the preparation for future outbreaks. This review describes the current state of the art for research of LAMP-based SARS-CoV-2 testing, as well as its implications for other pathogens and testing. The authors represent the global LAMP (gLAMP) Consortium, an international research collective, which has regularly met to share their experiences on LAMP deployment and best practices; sections are devoted to all aspects of LAMP testing, including preanalytic sample processing, target amplification, and amplicon detection, then the hardware and software required for deployment are discussed, and finally, a summary of the current regulatory landscape is provided. Included as well are a series of first-person accounts of LAMP method development and deployment. The final discussion section provides the reader with a distillation of the most validated testing methods and their paths to implementation. This review also aims to provide practical information and insight for a range of audiences: for a research audience, to help accelerate research through sharing of best practices; for an implementation audience, to help get testing up and running quickly; and for a public health, clinical, and policy audience, to help convey the breadth of the effect that LAMP methods have to offer. 2021-09-01T07:00:00Z text https://archium.ateneo.edu/chemistry-faculty-pubs/185 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802757/ Chemistry Faculty Publications Archīum Ateneo Diagnostics SARS-CoV2 point of care tests genomics loop-mediated isothermal amplification LAMP International Consortium Analytical, Diagnostic and Therapeutic Techniques and Equipment Chemistry
institution Ateneo De Manila University
building Ateneo De Manila University Library
continent Asia
country Philippines
Philippines
content_provider Ateneo De Manila University Library
collection archium.Ateneo Institutional Repository
topic Diagnostics
SARS-CoV2
point of care tests
genomics
loop-mediated isothermal amplification
LAMP
International Consortium
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Chemistry
spellingShingle Diagnostics
SARS-CoV2
point of care tests
genomics
loop-mediated isothermal amplification
LAMP
International Consortium
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Chemistry
Moore, Keith J M
Mason, Christopher E
co-authors, 56
Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications
description As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future outbreaks. The current gold standard for molecular diagnostics is the polymerase chain reaction (PCR), but the extraordinary and unmet demand for testing in a variety of environments means that both complementary and supplementary testing solutions are still needed. This review highlights the role that loop-mediated isothermal amplification (LAMP) has had in filling this global testing need, providing a faster and easier means of testing, and what it can do for future applications, pathogens, and the preparation for future outbreaks. This review describes the current state of the art for research of LAMP-based SARS-CoV-2 testing, as well as its implications for other pathogens and testing. The authors represent the global LAMP (gLAMP) Consortium, an international research collective, which has regularly met to share their experiences on LAMP deployment and best practices; sections are devoted to all aspects of LAMP testing, including preanalytic sample processing, target amplification, and amplicon detection, then the hardware and software required for deployment are discussed, and finally, a summary of the current regulatory landscape is provided. Included as well are a series of first-person accounts of LAMP method development and deployment. The final discussion section provides the reader with a distillation of the most validated testing methods and their paths to implementation. This review also aims to provide practical information and insight for a range of audiences: for a research audience, to help accelerate research through sharing of best practices; for an implementation audience, to help get testing up and running quickly; and for a public health, clinical, and policy audience, to help convey the breadth of the effect that LAMP methods have to offer.
format text
author Moore, Keith J M
Mason, Christopher E
co-authors, 56
author_facet Moore, Keith J M
Mason, Christopher E
co-authors, 56
author_sort Moore, Keith J M
title Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications
title_short Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications
title_full Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications
title_fullStr Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications
title_full_unstemmed Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications
title_sort loop-mediated isothermal amplification detection of sars-cov-2 and myriad other applications
publisher Archīum Ateneo
publishDate 2021
url https://archium.ateneo.edu/chemistry-faculty-pubs/185
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802757/
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