Development of 31P Nuclear Magnetic Resonance Methods for the Study of Phosphate Metabolisms in E. coli and B. subtilis

• Main Authors: Dayrit, Fabian M, Espiritu, Emilyn Q, Gonzalez, Noreen, Rojas, Nina Rosario L, Aguilan, Jennifer T, Basilio, Antonio M, Chainani, Edward T, Cruz, Enrico, Matanguihan, Bernadette
• Format: text
• Published: Archīum Ateneo 1999
• Subjects: 31P NMR method; phosphate metabolism; E. coli; B. subtilis; Biochemistry; Molecular Biology
• Online Access: https://archium.ateneo.edu/es-faculty-pubs/59; https://archium.ateneo.edu/cgi/viewcontent.cgi?article=1058&context=es-faculty-pubs
• Institution: Ateneo De Manila University

31P NMR experiments were performed on Escherichia coli and Bacillus subtilis at various temperatures under aerobic and anaerobic conditions. The total soluble intracellular phosphate concentration was estimated to be 2 x 10^-17 mole/cell, while intracellular orthophosphate concentration was around 1...