Development of 31P Nuclear Magnetic Resonance Methods for the Study of Phosphate Metabolisms in E. coli and B. subtilis

• Main Authors: Dayrit, Fabian M, Espiritu, Emilyn Q, Gonzalez, Noreen, Rojas, Nina Rosario L, Aguilan, Jennifer T, Basilio, Antonio M, Chainani, Edward T, Cruz, Enrico, Matanguihan, Bernadette
• 格式: text
• 出版: Archīum Ateneo 1999
• 主題: 31P NMR method; phosphate metabolism; E. coli; B. subtilis; Biochemistry; Molecular Biology
• 在線閱讀: https://archium.ateneo.edu/es-faculty-pubs/59; https://archium.ateneo.edu/cgi/viewcontent.cgi?article=1058&context=es-faculty-pubs

31P NMR experiments were performed on Escherichia coli and Bacillus subtilis at various temperatures under aerobic and anaerobic conditions. The total soluble intracellular phosphate concentration was estimated to be 2 x 10^-17 mole/cell, while intracellular orthophosphate concentration was around 1...