Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered saccharomyces cerevisiae
Production of ethanol and xylitol from lignocellulosic hydrolysates is an alternative to the traditional production of ethanol in utilizing biomass. However, the conversion efficiency of xylose to xylitol is restricted by glucose repression, causing a low xylitol titer. To this end, we cloned genes...
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sg-ntu-dr.10356-1002972023-12-29T06:49:24Z Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered saccharomyces cerevisiae Zha, Jian Li, Bing Zhi Shen, Ming Hua Hu, Meng Long Song, Hao Yuan, Ying Jin Du, Chenyu School of Chemical and Biomedical Engineering Chemical and Biomedical Engineering Production of ethanol and xylitol from lignocellulosic hydrolysates is an alternative to the traditional production of ethanol in utilizing biomass. However, the conversion efficiency of xylose to xylitol is restricted by glucose repression, causing a low xylitol titer. To this end, we cloned genes CDT-1 (encoding a cellodextrin transporter) and gh1-1 (encoding an intracellular β-glucosidase) from Neurospora crassa and XYL1 (encoding a xylose reductase that converts xylose into xylitol) from Scheffersomyces stipitis into Saccharomyces cerevisiae, enabling simultaneous production of ethanol and xylitol from a mixture of cellobiose and xylose (main components of lignocellulosic hydrolysates). We further optimized the expression levels of CDT-1 and XYL1 by manipulating their promoters and copy-numbers, and constructed an engineered S. cerevisiae strain (carrying one copy of PGK1p-CDT1 and two copies of TDH3p-XYL1), which showed an 85.7% increase in xylitol production from the mixture of cellobiose and xylose than that from the mixture of glucose and xylose. Thus, we achieved a balanced co-fermentation of cellobiose (0.165 g/L/h) and xylose (0.162 g/L/h) at similar rates to co-produce ethanol (0.36 g/g) and xylitol (1.00 g/g). Published version 2014-01-20T04:28:55Z 2019-12-06T20:19:58Z 2014-01-20T04:28:55Z 2019-12-06T20:19:58Z 2013 2013 Journal Article Zha, J., Li, B. Z., Shen, M. H., Hu, M. L., Song, H., & Yuan, Y. J. (2013). Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered saccharomyces cerevisiae. PLoS ONE, 8(7), e68317-. 1932-6203 https://hdl.handle.net/10356/100297 http://hdl.handle.net/10220/18628 10.1371/journal.pone.0068317 23844185 en PLoS ONE © 2013 The Author(s). This paper was published in PLoS ONE and is made available as an electronic reprint (preprint) with permission of The Author(s). . The paper can be found at the following official DOI: [http://dx.doi.org/10.1371/journal.pone.0068317]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf |
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Chemical and Biomedical Engineering Zha, Jian Li, Bing Zhi Shen, Ming Hua Hu, Meng Long Song, Hao Yuan, Ying Jin Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered saccharomyces cerevisiae |
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Production of ethanol and xylitol from lignocellulosic hydrolysates is an alternative to the traditional production of ethanol in utilizing biomass. However, the conversion efficiency of xylose to xylitol is restricted by glucose repression, causing a low xylitol titer. To this end, we cloned genes CDT-1 (encoding a cellodextrin transporter) and gh1-1 (encoding an intracellular β-glucosidase) from Neurospora crassa and XYL1 (encoding a xylose reductase that converts xylose into xylitol) from Scheffersomyces stipitis into Saccharomyces cerevisiae, enabling simultaneous production of ethanol and xylitol from a mixture of cellobiose and xylose (main components of lignocellulosic hydrolysates). We further optimized the expression levels of CDT-1 and XYL1 by manipulating their promoters and copy-numbers, and constructed an engineered S. cerevisiae strain (carrying one copy of PGK1p-CDT1 and two copies of TDH3p-XYL1), which showed an 85.7% increase in xylitol production from the mixture of cellobiose and xylose than that from the mixture of glucose and xylose. Thus, we achieved a balanced co-fermentation of cellobiose (0.165 g/L/h) and xylose (0.162 g/L/h) at similar rates to co-produce ethanol (0.36 g/g) and xylitol (1.00 g/g). |
author2 |
Du, Chenyu |
author_facet |
Du, Chenyu Zha, Jian Li, Bing Zhi Shen, Ming Hua Hu, Meng Long Song, Hao Yuan, Ying Jin |
format |
Article |
author |
Zha, Jian Li, Bing Zhi Shen, Ming Hua Hu, Meng Long Song, Hao Yuan, Ying Jin |
author_sort |
Zha, Jian |
title |
Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered saccharomyces cerevisiae |
title_short |
Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered saccharomyces cerevisiae |
title_full |
Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered saccharomyces cerevisiae |
title_fullStr |
Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered saccharomyces cerevisiae |
title_full_unstemmed |
Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered saccharomyces cerevisiae |
title_sort |
optimization of cdt-1 and xyl1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered saccharomyces cerevisiae |
publishDate |
2014 |
url |
https://hdl.handle.net/10356/100297 http://hdl.handle.net/10220/18628 |
_version_ |
1787136602144768000 |