Assessment of plant-driven uptake and translocation of clofibric acid by Scirpus validus

Pharmaceutical compounds are now considered as emerging contaminants of environmental concern. The overall objective of this study was to evaluate the uptake and translocation of clofibric acid (CA) by the macrophyte Scirpus validus growing hydroponically. A set of the three replicates was establish...

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Main Authors: Zhang, Dong Qing, Gersberg, Richard M., Hua, Tao, Zhu, Junfei, Ng, Wun Jern, Tan, Soon Keat
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/100523
http://hdl.handle.net/10220/16240
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1005232020-03-07T11:43:40Z Assessment of plant-driven uptake and translocation of clofibric acid by Scirpus validus Zhang, Dong Qing Gersberg, Richard M. Hua, Tao Zhu, Junfei Ng, Wun Jern Tan, Soon Keat School of Civil and Environmental Engineering Nanyang Environment and Water Research Institute Maritime Research Centre DRNTU::Engineering::Civil engineering DRNTU::Engineering::Environmental engineering Pharmaceutical compounds are now considered as emerging contaminants of environmental concern. The overall objective of this study was to evaluate the uptake and translocation of clofibric acid (CA) by the macrophyte Scirpus validus growing hydroponically. A set of the three replicates was established for each exposure time and for each CA concentration. Plants were grown in 4 L vessels (four plants per vessel corresponding to the three exposure period studies, i.e., 7, 14, 18, and 21 days) which contained an aerated modified Hoagland nutrient solution that was spiked with CA at concentrations of 0.5, 1.0, and 2.0 mg L−1. At each exposure period, CA concentration was measured in the nutrient solutions. A sea sand disruption method was employed for the extraction of CA from plant tissues. The determination of the pharmaceutical concentration was carried out using solid phase extraction (SPE) followed by chromatographic analysis. The quantification of CA concentrations in both nutrient solutions (after SPE) and plant tissues (after extraction) was conducted by chromatographic analysis. CA concentrations of 5.4–26.8 μg g−1 (fresh weight) were detected in the roots and 7.2–34.6 μg g−1 (fresh weight) in the shoots after 21 days. Mass balance calculations showed that S. validus uptake alone accounted for a significant contribution (6–13 % for the roots and 22–49 % for the shoots) of the total loss of CA. The bioaccumulation factors (BAFs) based on fresh weight for the roots ranged from 6.6 to 23.2, while values for the shoots ranged from 9.5 to 32.1. All the BAFs for the shoots were greater than those in the roots, implying that CA has greater tendency to be translocated to the shoots, rather than the roots of S. validus. All the shoot-to-root concentration ratios were more than 1, denoting that the shoots of S. validus do preferentially accumulate CA. We demonstrated that CA can be actively taken up, subsequently translocated and accumulated by aboveground tissues of S. validus. Since S. validus could account for the removal of 28–62 % of the total mass loss of CA from the system, such phytoremediation technology has great potential for the removal of pharmaceuticals such as CA from inflowing waters. 2013-10-04T01:38:02Z 2019-12-06T20:24:01Z 2013-10-04T01:38:02Z 2019-12-06T20:24:01Z 2012 2012 Journal Article Zhang, D. Q., Gersberg, R. M., Hua, T., Zhu, J., Ng, W. J., & Tan, S. K. (2012). Assessment of plant-driven uptake and translocation of clofibric acid by Scirpus validus. Environmental science and pollution research, 20(7), 4612-4620. https://hdl.handle.net/10356/100523 http://hdl.handle.net/10220/16240 10.1007/s11356-012-1375-1 en Environmental science and pollution research
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Civil engineering
DRNTU::Engineering::Environmental engineering
spellingShingle DRNTU::Engineering::Civil engineering
DRNTU::Engineering::Environmental engineering
Zhang, Dong Qing
Gersberg, Richard M.
Hua, Tao
Zhu, Junfei
Ng, Wun Jern
Tan, Soon Keat
Assessment of plant-driven uptake and translocation of clofibric acid by Scirpus validus
description Pharmaceutical compounds are now considered as emerging contaminants of environmental concern. The overall objective of this study was to evaluate the uptake and translocation of clofibric acid (CA) by the macrophyte Scirpus validus growing hydroponically. A set of the three replicates was established for each exposure time and for each CA concentration. Plants were grown in 4 L vessels (four plants per vessel corresponding to the three exposure period studies, i.e., 7, 14, 18, and 21 days) which contained an aerated modified Hoagland nutrient solution that was spiked with CA at concentrations of 0.5, 1.0, and 2.0 mg L−1. At each exposure period, CA concentration was measured in the nutrient solutions. A sea sand disruption method was employed for the extraction of CA from plant tissues. The determination of the pharmaceutical concentration was carried out using solid phase extraction (SPE) followed by chromatographic analysis. The quantification of CA concentrations in both nutrient solutions (after SPE) and plant tissues (after extraction) was conducted by chromatographic analysis. CA concentrations of 5.4–26.8 μg g−1 (fresh weight) were detected in the roots and 7.2–34.6 μg g−1 (fresh weight) in the shoots after 21 days. Mass balance calculations showed that S. validus uptake alone accounted for a significant contribution (6–13 % for the roots and 22–49 % for the shoots) of the total loss of CA. The bioaccumulation factors (BAFs) based on fresh weight for the roots ranged from 6.6 to 23.2, while values for the shoots ranged from 9.5 to 32.1. All the BAFs for the shoots were greater than those in the roots, implying that CA has greater tendency to be translocated to the shoots, rather than the roots of S. validus. All the shoot-to-root concentration ratios were more than 1, denoting that the shoots of S. validus do preferentially accumulate CA. We demonstrated that CA can be actively taken up, subsequently translocated and accumulated by aboveground tissues of S. validus. Since S. validus could account for the removal of 28–62 % of the total mass loss of CA from the system, such phytoremediation technology has great potential for the removal of pharmaceuticals such as CA from inflowing waters.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Zhang, Dong Qing
Gersberg, Richard M.
Hua, Tao
Zhu, Junfei
Ng, Wun Jern
Tan, Soon Keat
format Article
author Zhang, Dong Qing
Gersberg, Richard M.
Hua, Tao
Zhu, Junfei
Ng, Wun Jern
Tan, Soon Keat
author_sort Zhang, Dong Qing
title Assessment of plant-driven uptake and translocation of clofibric acid by Scirpus validus
title_short Assessment of plant-driven uptake and translocation of clofibric acid by Scirpus validus
title_full Assessment of plant-driven uptake and translocation of clofibric acid by Scirpus validus
title_fullStr Assessment of plant-driven uptake and translocation of clofibric acid by Scirpus validus
title_full_unstemmed Assessment of plant-driven uptake and translocation of clofibric acid by Scirpus validus
title_sort assessment of plant-driven uptake and translocation of clofibric acid by scirpus validus
publishDate 2013
url https://hdl.handle.net/10356/100523
http://hdl.handle.net/10220/16240
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