Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization

Proteomics analysis of lignocellulolytic proteins by lignocellulosic biomass degrading microbes and compatible microbial consortium is a promising approach that offers a new means to enzyme discovery. The abundance of proteins in complex secretome by microbial communities would highlight key lignoce...

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Main Authors: Ravindran, Anita, Cheow, Esther Sok Hwee, Sze, Siu Kwan, Adav, Sunil S.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/100744
http://hdl.handle.net/10220/13688
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1007442020-03-07T12:24:53Z Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization Ravindran, Anita Cheow, Esther Sok Hwee Sze, Siu Kwan Adav, Sunil S. School of Biological Sciences DRNTU::Science::Biological sciences Proteomics analysis of lignocellulolytic proteins by lignocellulosic biomass degrading microbes and compatible microbial consortium is a promising approach that offers a new means to enzyme discovery. The abundance of proteins in complex secretome by microbial communities would highlight key lignocellulolytic proteins for lignocellulosic biorefinery. In this study, lignocellulolytic enzymes of potent lignin degrading basidiomycota and effective cellulolytic ascomycota fungal strains, and their co-cultures were analyzed using high throughput isobaric tag for relative and absolute quantitation (iTRAQ) technique using liquid chromatography tandem mass spectrometry. Protein abundances in the iTRAQ-multiplexed samples were determined by integrating relative quantitation and exponentially modified protein abundance index (emPAI). The functional classification of the secretory proteins by individual culture and co-culture demonstrated 36.77% cellulolytic proteins, 13.06% hemicellulases, 14.09% ligninolytic proteins, 19.59% proteolytic enzymes. 7.22% hypothetical proteins and 6.87% cell morphogenesis proteins. The abundance of the proteins by individual cultures and co-cultured fungal consortium revealed that co-culturing of Phanerochaete chrysosporium with Trichoderma reesei QM6a and Trichoderma reesei Rut C30 induced the production of cellulolytic proteins and stimulated expression of hemicellulolytic enzymes. The hierarchical clustering of proteins in secretome of fungal strains and their co-cultures elucidated differential expressions of lignocellulolytic proteins by the microbial consortium. 2013-09-25T06:51:41Z 2019-12-06T20:27:29Z 2013-09-25T06:51:41Z 2019-12-06T20:27:29Z 2012 2012 Journal Article Adav, S. S., Ravindran, A., Cheow, E. S. H., & Sze, S. K. (2012). Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization. Journal of Proteomics, 75(18), 5590–5603. https://hdl.handle.net/10356/100744 http://hdl.handle.net/10220/13688 10.1016/j.jprot.2012.08.011 en Journal of proteomics
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Ravindran, Anita
Cheow, Esther Sok Hwee
Sze, Siu Kwan
Adav, Sunil S.
Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization
description Proteomics analysis of lignocellulolytic proteins by lignocellulosic biomass degrading microbes and compatible microbial consortium is a promising approach that offers a new means to enzyme discovery. The abundance of proteins in complex secretome by microbial communities would highlight key lignocellulolytic proteins for lignocellulosic biorefinery. In this study, lignocellulolytic enzymes of potent lignin degrading basidiomycota and effective cellulolytic ascomycota fungal strains, and their co-cultures were analyzed using high throughput isobaric tag for relative and absolute quantitation (iTRAQ) technique using liquid chromatography tandem mass spectrometry. Protein abundances in the iTRAQ-multiplexed samples were determined by integrating relative quantitation and exponentially modified protein abundance index (emPAI). The functional classification of the secretory proteins by individual culture and co-culture demonstrated 36.77% cellulolytic proteins, 13.06% hemicellulases, 14.09% ligninolytic proteins, 19.59% proteolytic enzymes. 7.22% hypothetical proteins and 6.87% cell morphogenesis proteins. The abundance of the proteins by individual cultures and co-cultured fungal consortium revealed that co-culturing of Phanerochaete chrysosporium with Trichoderma reesei QM6a and Trichoderma reesei Rut C30 induced the production of cellulolytic proteins and stimulated expression of hemicellulolytic enzymes. The hierarchical clustering of proteins in secretome of fungal strains and their co-cultures elucidated differential expressions of lignocellulolytic proteins by the microbial consortium.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Ravindran, Anita
Cheow, Esther Sok Hwee
Sze, Siu Kwan
Adav, Sunil S.
format Article
author Ravindran, Anita
Cheow, Esther Sok Hwee
Sze, Siu Kwan
Adav, Sunil S.
author_sort Ravindran, Anita
title Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization
title_short Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization
title_full Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization
title_fullStr Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization
title_full_unstemmed Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization
title_sort quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization
publishDate 2013
url https://hdl.handle.net/10356/100744
http://hdl.handle.net/10220/13688
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