Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging

This study provides a successful preparation of biocompatible hybrid materials (1-GO and 2-GO) by the integration of graphene oxide (GO) with water-soluble pillararenes (bolaamphiphile 1 and tadpolelike amphiphile 2) for dual-mode Raman and fluorescence bioimaging in vitro. The investigations show t...

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Main Authors: Zhao, Yanli, Zhang, Huacheng, Ma, Xing, Nguyen, Kim Truc, Zeng, Yongfei, Tai, Shuhui
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/100962
http://hdl.handle.net/10220/19691
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1009622020-06-01T10:26:49Z Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging Zhao, Yanli Zhang, Huacheng Ma, Xing Nguyen, Kim Truc Zeng, Yongfei Tai, Shuhui School of Materials Science & Engineering School of Physical and Mathematical Sciences DRNTU::Engineering::Materials::Biomaterials This study provides a successful preparation of biocompatible hybrid materials (1-GO and 2-GO) by the integration of graphene oxide (GO) with water-soluble pillararenes (bolaamphiphile 1 and tadpolelike amphiphile 2) for dual-mode Raman and fluorescence bioimaging in vitro. The investigations show that pillararenes 1 and 2 were loaded onto the surface of GO through strong hydrogen-bonding interactions. Aqueous suspensions of 1-GO and 2-GO are stable and can be kept for a long time. After confirming their good biocompatibility by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the 1-GO and 2-GO hybrids were endocytosed by HeLa cells for in vitro Raman imaging. It was found that 1-GO presents better Raman imaging than 2-GO. When a fluorescent guest molecule, bipyridinium derivative 3, was added into the suspensions of the hybrids, the suspensions of 1-GO and 2-GO were as stable as the original. The suspensions of the inclusion complexes (1-GO[DOT OPERATOR]3 and 2-GO[DOT OPERATOR]3) formed from 1-GO and 2-GO with 3 can also be endocytosed by HeLa cells to enable in vitro fluorescence imaging to be performed. It was found that 1-GO[DOT OPERATOR]3 performs better than 2-GO[DOT OPERATOR]3. The current research has determined the capacities of pillararene-modified GO for combined bioimaging, which paves the way for using these biocompatible materials towards dual-mode diagnostics. 2014-06-12T03:42:28Z 2019-12-06T20:31:29Z 2014-06-12T03:42:28Z 2019-12-06T20:31:29Z 2014 2014 Journal Article Zhang, H., Ma, X., Nguyen, K. T., Zeng, Y., Tai, S., & Zhao, Y. (2014). Water-Soluble Pillararene-Functionalized Graphene Oxide for In Vitro Raman and Fluorescence Dual-Mode Imaging. ChemPlusChem, 79(3), 462-469. 2192-6506 https://hdl.handle.net/10356/100962 http://hdl.handle.net/10220/19691 10.1002/cplu.201300408 en ChemPlusChem © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
spellingShingle DRNTU::Engineering::Materials::Biomaterials
Zhao, Yanli
Zhang, Huacheng
Ma, Xing
Nguyen, Kim Truc
Zeng, Yongfei
Tai, Shuhui
Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging
description This study provides a successful preparation of biocompatible hybrid materials (1-GO and 2-GO) by the integration of graphene oxide (GO) with water-soluble pillararenes (bolaamphiphile 1 and tadpolelike amphiphile 2) for dual-mode Raman and fluorescence bioimaging in vitro. The investigations show that pillararenes 1 and 2 were loaded onto the surface of GO through strong hydrogen-bonding interactions. Aqueous suspensions of 1-GO and 2-GO are stable and can be kept for a long time. After confirming their good biocompatibility by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the 1-GO and 2-GO hybrids were endocytosed by HeLa cells for in vitro Raman imaging. It was found that 1-GO presents better Raman imaging than 2-GO. When a fluorescent guest molecule, bipyridinium derivative 3, was added into the suspensions of the hybrids, the suspensions of 1-GO and 2-GO were as stable as the original. The suspensions of the inclusion complexes (1-GO[DOT OPERATOR]3 and 2-GO[DOT OPERATOR]3) formed from 1-GO and 2-GO with 3 can also be endocytosed by HeLa cells to enable in vitro fluorescence imaging to be performed. It was found that 1-GO[DOT OPERATOR]3 performs better than 2-GO[DOT OPERATOR]3. The current research has determined the capacities of pillararene-modified GO for combined bioimaging, which paves the way for using these biocompatible materials towards dual-mode diagnostics.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Zhao, Yanli
Zhang, Huacheng
Ma, Xing
Nguyen, Kim Truc
Zeng, Yongfei
Tai, Shuhui
format Article
author Zhao, Yanli
Zhang, Huacheng
Ma, Xing
Nguyen, Kim Truc
Zeng, Yongfei
Tai, Shuhui
author_sort Zhao, Yanli
title Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging
title_short Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging
title_full Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging
title_fullStr Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging
title_full_unstemmed Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging
title_sort water-soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging
publishDate 2014
url https://hdl.handle.net/10356/100962
http://hdl.handle.net/10220/19691
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