Label-free virus identification and characterization using electrochemical impedance spectroscopy

We demonstrate here the application of electrochemical impedance spectroscopy (EIS) in microfluidic devices for label-free virus identification by means of their specific “signature” and also investigate its feasibility for titer quantitation using two basic approaches. The first one is a method bas...

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Main Authors: Poenar, Daniel P., Iliescu, Ciprian, Boulaire, Jérôme, Yu, Hanry
Other Authors: School of Electrical and Electronic Engineering
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/100972
http://hdl.handle.net/10220/19023
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1009722020-03-07T14:00:34Z Label-free virus identification and characterization using electrochemical impedance spectroscopy Poenar, Daniel P. Iliescu, Ciprian Boulaire, Jérôme Yu, Hanry School of Electrical and Electronic Engineering DRNTU::Engineering::Electrical and electronic engineering::Electronic systems::Signal processing We demonstrate here the application of electrochemical impedance spectroscopy (EIS) in microfluidic devices for label-free virus identification by means of their specific “signature” and also investigate its feasibility for titer quantitation using two basic approaches. The first one is a method based on identifying so-called “resonance” frequencies manifesting in our microdevices and monitoring their variation as a function of the virus concentration, whereas the second one relies on measuring the relative impedance variation at these “resonance” frequencies. Best results have been obtained for the highest “resonance” frequency (∼80 MHz), which we attribute to be due to both the structure of the microdevice and the extremely small size of the viruses that make their effect significant only at such frequencies. This is a simpler method of determining virus concentration in diluted solutions of purified viruses than the well-established traditional plaque assay titer estimation method, and—since it is based on frequency measurement—could potentially be more accurate. 2014-03-28T06:14:31Z 2019-12-06T20:31:38Z 2014-03-28T06:14:31Z 2019-12-06T20:31:38Z 2013 2013 Journal Article Poenar, D. P., Iliescu, C., Boulaire, J., & Yu, H. (2014). Label-free virus identification and characterization using electrochemical impedance spectroscopy. ELECTROPHORESIS, 35(2-3), 433-440. 0173-0835 https://hdl.handle.net/10356/100972 http://hdl.handle.net/10220/19023 10.1002/elps.201300368 en ELECTROPHORESIS © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Electrical and electronic engineering::Electronic systems::Signal processing
spellingShingle DRNTU::Engineering::Electrical and electronic engineering::Electronic systems::Signal processing
Poenar, Daniel P.
Iliescu, Ciprian
Boulaire, Jérôme
Yu, Hanry
Label-free virus identification and characterization using electrochemical impedance spectroscopy
description We demonstrate here the application of electrochemical impedance spectroscopy (EIS) in microfluidic devices for label-free virus identification by means of their specific “signature” and also investigate its feasibility for titer quantitation using two basic approaches. The first one is a method based on identifying so-called “resonance” frequencies manifesting in our microdevices and monitoring their variation as a function of the virus concentration, whereas the second one relies on measuring the relative impedance variation at these “resonance” frequencies. Best results have been obtained for the highest “resonance” frequency (∼80 MHz), which we attribute to be due to both the structure of the microdevice and the extremely small size of the viruses that make their effect significant only at such frequencies. This is a simpler method of determining virus concentration in diluted solutions of purified viruses than the well-established traditional plaque assay titer estimation method, and—since it is based on frequency measurement—could potentially be more accurate.
author2 School of Electrical and Electronic Engineering
author_facet School of Electrical and Electronic Engineering
Poenar, Daniel P.
Iliescu, Ciprian
Boulaire, Jérôme
Yu, Hanry
format Article
author Poenar, Daniel P.
Iliescu, Ciprian
Boulaire, Jérôme
Yu, Hanry
author_sort Poenar, Daniel P.
title Label-free virus identification and characterization using electrochemical impedance spectroscopy
title_short Label-free virus identification and characterization using electrochemical impedance spectroscopy
title_full Label-free virus identification and characterization using electrochemical impedance spectroscopy
title_fullStr Label-free virus identification and characterization using electrochemical impedance spectroscopy
title_full_unstemmed Label-free virus identification and characterization using electrochemical impedance spectroscopy
title_sort label-free virus identification and characterization using electrochemical impedance spectroscopy
publishDate 2014
url https://hdl.handle.net/10356/100972
http://hdl.handle.net/10220/19023
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