Early detection of dengue virus by use of reverse transcription-recombinase polymerase amplification

• Main Authors: Teoh, Boon-Teong, Sam, Sing-Sin, Tan, Kim-Kee, Danlami, Mohammed Bashar, Shu, Meng-Hooi, Johari, Jefree, Hooi, Poh-Sim, Brooks, David, Piepenburg, Olaf, Nentwich, Oliver, Wilder-Smith, Annelies, Franco, Leticia, Tenorio, Antonio, AbuBakar, Sazaly
• Other Authors: Tang, Y.-W.
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Biological sciences::Microbiology
• Online Access: https://hdl.handle.net/10356/101099; http://hdl.handle.net/10220/25718
• Institution: Nanyang Technological University

A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay e...