Biomimetic nanocomposites to control osteogenic differentiation of human mesenchymal stem cells
The design of biomimetic nanomaterials that can directly influence the behavior of cells and facilitate the regeneration of tissues and organs has become an active area of research. Here, the production of materials based on nano-hydroxyapatite composites in scaffolds with nanofibrous and nanoporous...
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sg-ntu-dr.10356-1011002020-06-01T10:01:37Z Biomimetic nanocomposites to control osteogenic differentiation of human mesenchymal stem cells Liao, Susan Nguyen, Luong T. H. Ngiam, Michelle Wang, Charlene Cheng, Ziyuan Chan, Casey K. Ramakrishna, Seeram School of Materials Science & Engineering DRNTU::Engineering::Materials::Composite materials The design of biomimetic nanomaterials that can directly influence the behavior of cells and facilitate the regeneration of tissues and organs has become an active area of research. Here, the production of materials based on nano-hydroxyapatite composites in scaffolds with nanofibrous and nanoporous topographies, designed to mimic the native bone matrix for applications in bone tissue engineering, is reported. Human mesenchymal stem cells grown on these nanocomposites are stimulated to rapidly produce bone minerals in situ, even in the absence of osteogenic supplements in the cell-culture medium. Nanocomposites comprising type I collagen and nano-hydroxyapatite are found to be especially efficient at inducing mineralization. When subcutaneously implanted into nude mice, this biomimetic nanocomposite is able to form a new bone matrix within only two weeks. Furthermore, when the nanocomposite is enriched with human mesenchymal stem cells before implantation, development of the bone matrix is accelerated to within one week. To the best of the authors' knowledge, this study provides the first clear in vitro and in vivo demonstration of osteoinduction controlled by the material characteristics of a biomimetic nanocomposite. This approach can potentially facilitate the translation of de novo bone-formation technologies to the clinic. 2014-06-12T08:46:14Z 2019-12-06T20:33:24Z 2014-06-12T08:46:14Z 2019-12-06T20:33:24Z 2013 2013 Journal Article Liao, S., Nguyen, L. T. H., Ngiam, M., Wang, C., Cheng, Z., Chan, C. K., et al. (2014). Biomimetic Nanocomposites to Control Osteogenic Differentiation of Human Mesenchymal Stem Cells. Advanced Healthcare Materials, 3(5), 737-751. 2192-2640 https://hdl.handle.net/10356/101100 http://hdl.handle.net/10220/19717 10.1002/adhm.201300207 en Advanced healthcare materials © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
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DRNTU::Engineering::Materials::Composite materials Liao, Susan Nguyen, Luong T. H. Ngiam, Michelle Wang, Charlene Cheng, Ziyuan Chan, Casey K. Ramakrishna, Seeram Biomimetic nanocomposites to control osteogenic differentiation of human mesenchymal stem cells |
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The design of biomimetic nanomaterials that can directly influence the behavior of cells and facilitate the regeneration of tissues and organs has become an active area of research. Here, the production of materials based on nano-hydroxyapatite composites in scaffolds with nanofibrous and nanoporous topographies, designed to mimic the native bone matrix for applications in bone tissue engineering, is reported. Human mesenchymal stem cells grown on these nanocomposites are stimulated to rapidly produce bone minerals in situ, even in the absence of osteogenic supplements in the cell-culture medium. Nanocomposites comprising type I collagen and nano-hydroxyapatite are found to be especially efficient at inducing mineralization. When subcutaneously implanted into nude mice, this biomimetic nanocomposite is able to form a new bone matrix within only two weeks. Furthermore, when the nanocomposite is enriched with human mesenchymal stem cells before implantation, development of the bone matrix is accelerated to within one week. To the best of the authors' knowledge, this study provides the first clear in vitro and in vivo demonstration of osteoinduction controlled by the material characteristics of a biomimetic nanocomposite. This approach can potentially facilitate the translation of de novo bone-formation technologies to the clinic. |
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School of Materials Science & Engineering |
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School of Materials Science & Engineering Liao, Susan Nguyen, Luong T. H. Ngiam, Michelle Wang, Charlene Cheng, Ziyuan Chan, Casey K. Ramakrishna, Seeram |
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Article |
author |
Liao, Susan Nguyen, Luong T. H. Ngiam, Michelle Wang, Charlene Cheng, Ziyuan Chan, Casey K. Ramakrishna, Seeram |
author_sort |
Liao, Susan |
title |
Biomimetic nanocomposites to control osteogenic differentiation of human mesenchymal stem cells |
title_short |
Biomimetic nanocomposites to control osteogenic differentiation of human mesenchymal stem cells |
title_full |
Biomimetic nanocomposites to control osteogenic differentiation of human mesenchymal stem cells |
title_fullStr |
Biomimetic nanocomposites to control osteogenic differentiation of human mesenchymal stem cells |
title_full_unstemmed |
Biomimetic nanocomposites to control osteogenic differentiation of human mesenchymal stem cells |
title_sort |
biomimetic nanocomposites to control osteogenic differentiation of human mesenchymal stem cells |
publishDate |
2014 |
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https://hdl.handle.net/10356/101100 http://hdl.handle.net/10220/19717 |
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1681059462913196032 |