Quantitative diagnosis of cervical neoplasia using fluorescence lifetime imaging on haematoxylin and eosin stained tissue sections

• Main Authors: Razul, Sirajudeen Gulam, Lim, Soo Kim, Gu, Jun, Fu, Chit Yaw, Ng, Beng Koon
• Other Authors: School of Electrical and Electronic Engineering
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Science::Biological sciences::Microbiology::Immunology
• Online Access: https://hdl.handle.net/10356/101495; http://hdl.handle.net/10220/24156
• Institution: Nanyang Technological University

The use of conventional fluorescence microscopy for characterizing tissue pathological states is limited by overlapping spectra and the dependence on excitation power and fluorophore concentration. Fluorescence lifetime imaging microscopy (FLIM) can overcome these limitations due to its insensitivit...