Reciprocal response of human oral epithelial cells to internalized silica nanoparticles

Silica nanoparticles (SiO2 NPs) are one of the most widely used engineered nanoparticles and can been found in a wide range of consumer products. Despite their massive global production scale, little is known about their potential effects in the context of unintended exposure or ingestion. Using TR1...

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Main Authors: Tay, Chor Yong, Fang, Wanru, Setyawati, Magdiel Inggrid, Sum, Chee Peng, Xie, Jianping, Ng, Kee Woei, Chen, Xiaodong, Hong, Catherine Hsu Ling, Leong, David Tai
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/101697
http://hdl.handle.net/10220/19791
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1016972020-06-01T10:21:24Z Reciprocal response of human oral epithelial cells to internalized silica nanoparticles Tay, Chor Yong Fang, Wanru Setyawati, Magdiel Inggrid Sum, Chee Peng Xie, Jianping Ng, Kee Woei Chen, Xiaodong Hong, Catherine Hsu Ling Leong, David Tai School of Materials Science & Engineering DRNTU::Engineering::Materials::Nanostructured materials Silica nanoparticles (SiO2 NPs) are one of the most widely used engineered nanoparticles and can been found in a wide range of consumer products. Despite their massive global production scale, little is known about their potential effects in the context of unintended exposure or ingestion. Using TR146 cells as an in vitro model of the human oral buccal mucosa, the uptake, spatial intracellular distribution, reactive oxygen species (ROS) production, inflammatory response, and cytotoxic effects of commercial SiO2 NPs are examined. SiO2 NPs are shown to dock and cross the cellular membrane barrier in a dose–time-dependent manner. Confocal sectioning reveals translocation of SiO2 NPs into the cell nucleus after 12 h of exposure. A concentration threshold of more than 500 × 10−6 m is observed, above which SiO2 NPs are shown to exert significant oxidative stress with concomitant upregulation of inflammatory genes IL6 and TNFA. Further analysis of the p53 pathway and a series of apoptotic and cell cycle biomarkers reveals intracellular accumulation of SiO2 NPs exert marginal nanotoxicity. Collectively, this study provides important information regarding the uptake, intracellular distribution, and potential adverse cellular effects of SiO2 NPs commonly found in consumer products in the human oral epithelium. 2014-06-16T04:04:34Z 2019-12-06T20:42:58Z 2014-06-16T04:04:34Z 2019-12-06T20:42:58Z 2013 2013 Journal Article Tay, C. Y., Fang, W., Setyawati, M. I., Sum, C. P., Xie, J., Ng, K. W., et al. (2013). Reciprocal Response of Human Oral Epithelial Cells to Internalized Silica Nanoparticles. Particle & Particle Systems Characterization, 30(9), 784-793. 0934-0866 https://hdl.handle.net/10356/101697 http://hdl.handle.net/10220/19791 10.1002/ppsc.201300111 en Particle & particle systems characterization © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Nanostructured materials
spellingShingle DRNTU::Engineering::Materials::Nanostructured materials
Tay, Chor Yong
Fang, Wanru
Setyawati, Magdiel Inggrid
Sum, Chee Peng
Xie, Jianping
Ng, Kee Woei
Chen, Xiaodong
Hong, Catherine Hsu Ling
Leong, David Tai
Reciprocal response of human oral epithelial cells to internalized silica nanoparticles
description Silica nanoparticles (SiO2 NPs) are one of the most widely used engineered nanoparticles and can been found in a wide range of consumer products. Despite their massive global production scale, little is known about their potential effects in the context of unintended exposure or ingestion. Using TR146 cells as an in vitro model of the human oral buccal mucosa, the uptake, spatial intracellular distribution, reactive oxygen species (ROS) production, inflammatory response, and cytotoxic effects of commercial SiO2 NPs are examined. SiO2 NPs are shown to dock and cross the cellular membrane barrier in a dose–time-dependent manner. Confocal sectioning reveals translocation of SiO2 NPs into the cell nucleus after 12 h of exposure. A concentration threshold of more than 500 × 10−6 m is observed, above which SiO2 NPs are shown to exert significant oxidative stress with concomitant upregulation of inflammatory genes IL6 and TNFA. Further analysis of the p53 pathway and a series of apoptotic and cell cycle biomarkers reveals intracellular accumulation of SiO2 NPs exert marginal nanotoxicity. Collectively, this study provides important information regarding the uptake, intracellular distribution, and potential adverse cellular effects of SiO2 NPs commonly found in consumer products in the human oral epithelium.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Tay, Chor Yong
Fang, Wanru
Setyawati, Magdiel Inggrid
Sum, Chee Peng
Xie, Jianping
Ng, Kee Woei
Chen, Xiaodong
Hong, Catherine Hsu Ling
Leong, David Tai
format Article
author Tay, Chor Yong
Fang, Wanru
Setyawati, Magdiel Inggrid
Sum, Chee Peng
Xie, Jianping
Ng, Kee Woei
Chen, Xiaodong
Hong, Catherine Hsu Ling
Leong, David Tai
author_sort Tay, Chor Yong
title Reciprocal response of human oral epithelial cells to internalized silica nanoparticles
title_short Reciprocal response of human oral epithelial cells to internalized silica nanoparticles
title_full Reciprocal response of human oral epithelial cells to internalized silica nanoparticles
title_fullStr Reciprocal response of human oral epithelial cells to internalized silica nanoparticles
title_full_unstemmed Reciprocal response of human oral epithelial cells to internalized silica nanoparticles
title_sort reciprocal response of human oral epithelial cells to internalized silica nanoparticles
publishDate 2014
url https://hdl.handle.net/10356/101697
http://hdl.handle.net/10220/19791
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