Spectrophotometric study of fluorescence sensing and selective binding of biochemical substrates by 2,2'-bridged biso(β-cyclodextrin) and its water-soluble fullerene conjugate
A bis(β-cyclodextrin)-fullerene conjugate (3) linked at the secondary hydroxyl side was prepared in a good...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
2011
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/101875 http://hdl.handle.net/10220/6997 |
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| Institution: | Nanyang Technological University |
| Summary: | A bis(β-cyclodextrin)-fullerene conjugate (3) linked at the secondary hydroxyl side was prepared in a good
yield from its precursor N,N'-bis(2-(2-aminoethylamino)ethyl)malonamide-bridged bis(β-cyclodextrin) (2).
Spectrophotomeric studies on the conformation and the inclusion complexation behavior of 3 with a variety
of organic and biochemical substrates by means of UV-vis, FT-IR, NMR, fluorescence, and circular dichroism
spectroscopy showed that the bis(β-cyclodextrin)-fullerene conjugate displayed an intramolecular capsuletype
conformation in aqueous solution. Because of the multiple binding of bis(β-cyclodextrin) with substrates,
2 can act as an efficient fluorescence sensor for biochemical substrates, while its fullerene conjugate 3 displays
a capability of cleaving DNA under visible-light irradiation. |
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