Mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae

Fatty acids stored as triglycerides, an important source of cellular energy, are catabolized through β-oxidation pathways predicted to occur both in peroxisomes and mitochondria in filamentous fungi. Here, we characterize the function of Enoyl-CoA hydratase Ech1, a mitochondrial β-oxidation enzyme,...

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Main Authors: Patkar, Rajesh N., Ramos-Pamplona, Marilou., Gupta, Archna P., Fan, Yang., Naqvi, Naweed I.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/102095
http://hdl.handle.net/10220/11166
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1020952020-03-07T12:18:05Z Mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae Patkar, Rajesh N. Ramos-Pamplona, Marilou. Gupta, Archna P. Fan, Yang. Naqvi, Naweed I. School of Biological Sciences DRNTU::Science::Biological sciences Fatty acids stored as triglycerides, an important source of cellular energy, are catabolized through β-oxidation pathways predicted to occur both in peroxisomes and mitochondria in filamentous fungi. Here, we characterize the function of Enoyl-CoA hydratase Ech1, a mitochondrial β-oxidation enzyme, in the model phytopathogen Magnaporthe oryzae. Ech1 was found to be essential for conidial germination and viability of older hyphae. Unlike wild-type Magnaporthe, the ech1Δ failed to utilize C14 fatty acid and was partially impeded in growth on C16 and C18 fatty acids. Surprisingly, loss of β-oxidation led to significantly altered mitochondrial morphology and integrity with ech1Δ showing predominantly vesicular/punctate mitochondria in contrast to the fused tubular network in wild-type Magnaporthe. The ech1Δ appressoria were aberrant and displayed reduced melanization. Importantly, we show that the significantly reduced ability of ech1Δ to penetrate the host and establish therein is a direct consequence of enhanced sensitivity of the mutant to oxidative stress, as the defects could be remarkably reversed through exogenous antioxidants. Overall, our comparative analyses reveal that peroxisomal lipid catabolism is essential for appressorial function of host penetration, whereas mitochondrial β-oxidation primarily contributes to conidial viability and maintenance of redox homeostasis during host colonization by Magnaporthe. 2013-07-11T02:39:27Z 2019-12-06T20:49:40Z 2013-07-11T02:39:27Z 2019-12-06T20:49:40Z 2012 2012 Journal Article Patkar, R. N., Ramos-Pamplona, M., Gupta, A. P., Fan, Y., Naqvi, N. I. (2012). Mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae. Molecular Microbiology, 86(6), 1345-1363. https://hdl.handle.net/10356/102095 http://hdl.handle.net/10220/11166 10.1111/mmi.12060 en Molecular microbiology © 2012 Blackwell Publishing Ltd.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Patkar, Rajesh N.
Ramos-Pamplona, Marilou.
Gupta, Archna P.
Fan, Yang.
Naqvi, Naweed I.
Mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae
description Fatty acids stored as triglycerides, an important source of cellular energy, are catabolized through β-oxidation pathways predicted to occur both in peroxisomes and mitochondria in filamentous fungi. Here, we characterize the function of Enoyl-CoA hydratase Ech1, a mitochondrial β-oxidation enzyme, in the model phytopathogen Magnaporthe oryzae. Ech1 was found to be essential for conidial germination and viability of older hyphae. Unlike wild-type Magnaporthe, the ech1Δ failed to utilize C14 fatty acid and was partially impeded in growth on C16 and C18 fatty acids. Surprisingly, loss of β-oxidation led to significantly altered mitochondrial morphology and integrity with ech1Δ showing predominantly vesicular/punctate mitochondria in contrast to the fused tubular network in wild-type Magnaporthe. The ech1Δ appressoria were aberrant and displayed reduced melanization. Importantly, we show that the significantly reduced ability of ech1Δ to penetrate the host and establish therein is a direct consequence of enhanced sensitivity of the mutant to oxidative stress, as the defects could be remarkably reversed through exogenous antioxidants. Overall, our comparative analyses reveal that peroxisomal lipid catabolism is essential for appressorial function of host penetration, whereas mitochondrial β-oxidation primarily contributes to conidial viability and maintenance of redox homeostasis during host colonization by Magnaporthe.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Patkar, Rajesh N.
Ramos-Pamplona, Marilou.
Gupta, Archna P.
Fan, Yang.
Naqvi, Naweed I.
format Article
author Patkar, Rajesh N.
Ramos-Pamplona, Marilou.
Gupta, Archna P.
Fan, Yang.
Naqvi, Naweed I.
author_sort Patkar, Rajesh N.
title Mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae
title_short Mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae
title_full Mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae
title_fullStr Mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae
title_full_unstemmed Mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae
title_sort mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in magnaporthe oryzae
publishDate 2013
url https://hdl.handle.net/10356/102095
http://hdl.handle.net/10220/11166
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