A microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from low copy/single bacterial cells in minute sample volume followed by direct on-chip quantitative PCR assay

• Main Authors: Zhang, Rui, Gong Haiqing, Thomas, Zeng, Xu Dong, Lou, Chao-Ping, Sze, Chun Chau
• Other Authors: School of Mechanical and Aerospace Engineering
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Engineering::Mechanical engineering; DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/102378; http://hdl.handle.net/10220/16831
• Institution: Nanyang Technological University

Purification of nucleic acids from a low quantity of bacterial cells in minute volume is important in many clinical and biological applications. We developed a novel microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from bacterial cells in the range of 5000 d...