A high-throughput microfluidic biochip to quantify bacterial adhesion to single host cells by real-time PCR assay

• Main Authors: Zhang, Rui, Gong Haiqing, Thomas, Zeng, Xu Dong, Sze, Chun Chau
• Other Authors: School of Mechanical and Aerospace Engineering
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/102469; http://hdl.handle.net/10220/16867
• Institution: Nanyang Technological University

A high-throughput microfluidic poly-(dimethylsiloxane) biochip was developed to quantify bacterial adhesion to single host cells by real-time PCR assay. The biochip is simply structured with a two-dimensional array of 900 micro-wells, one inlet, and one outlet micro-channels. Isolation of single inf...