Enhancing E. coli isobutanol tolerance through engineering its global transcription factor cAMP receptor protein (CRP)

• Main Authors: Zhang, Hongfang, Song, Hao, Huang, Lei, Jiang, Rongrong, Chong, Huiqing, Geng, Hefang
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Engineering::Bioengineering
• Online Access: https://hdl.handle.net/10356/102792; http://hdl.handle.net/10220/19150
• Institution: Nanyang Technological University

The limited isobutanol tolerance of Escherichia coli is a major drawback during fermentative isobutanol production. Different from classical strain engineering approaches, this work was initiated to improve E. coli isobutanol tolerance from its transcriptional level by engineering its global transcr...