Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803

The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that “light” plasma membranes have a high carotenoid/pr...

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Main Authors: Zhang, Lifang, Selão, Tiago Toscano, Selstam, Eva, Norling, Birgitta
Other Authors: Chauvat, Franck
Format: Article
Language:English
Published: 2015
Online Access:https://hdl.handle.net/10356/103061
http://hdl.handle.net/10220/38702
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1030612023-02-28T17:04:42Z Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803 Zhang, Lifang Selão, Tiago Toscano Selstam, Eva Norling, Birgitta Chauvat, Franck School of Biological Sciences The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that “light” plasma membranes have a high carotenoid/protein ratio, when compared to “heavier” plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of β-carotene precursor molecules and echinenone. Published version 2015-09-17T05:15:11Z 2019-12-06T21:04:43Z 2015-09-17T05:15:11Z 2019-12-06T21:04:43Z 2015 2015 Journal Article Zhang, L., Selão, T. T., Selstam, E., & Norling, B. (2015). Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803. PLOS ONE, 10(6), e0130904-. 1932-6203 https://hdl.handle.net/10356/103061 http://hdl.handle.net/10220/38702 10.1371/journal.pone.0130904 26083372 en PLOS ONE © 2015 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
description The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that “light” plasma membranes have a high carotenoid/protein ratio, when compared to “heavier” plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of β-carotene precursor molecules and echinenone.
author2 Chauvat, Franck
author_facet Chauvat, Franck
Zhang, Lifang
Selão, Tiago Toscano
Selstam, Eva
Norling, Birgitta
format Article
author Zhang, Lifang
Selão, Tiago Toscano
Selstam, Eva
Norling, Birgitta
spellingShingle Zhang, Lifang
Selão, Tiago Toscano
Selstam, Eva
Norling, Birgitta
Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803
author_sort Zhang, Lifang
title Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803
title_short Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803
title_full Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803
title_fullStr Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803
title_full_unstemmed Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803
title_sort subcellular localization of carotenoid biosynthesis in synechocystis sp. pcc 6803
publishDate 2015
url https://hdl.handle.net/10356/103061
http://hdl.handle.net/10220/38702
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