Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure

Scaffolds providing a 3D environment which can effectively promote the adhesion, proliferation and differentiation of cells are crucial to tissue regeneration. In this study, the poly-l-lactic acid (PLLA) scaffold with hierarchical pore structural was fabricated via two-step thermally induced phase...

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Main Authors: Song, Kedong, Ji, Lili, Zhang, Jingying, Wang, Hai, Jiao, Zeren, Mayasari, Lim, Fu, Xiaoyan, Liu, Tianqing
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2015
Online Access:https://hdl.handle.net/10356/103362
http://hdl.handle.net/10220/38731
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1033622023-12-29T06:45:29Z Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure Song, Kedong Ji, Lili Zhang, Jingying Wang, Hai Jiao, Zeren Mayasari, Lim Fu, Xiaoyan Liu, Tianqing School of Chemical and Biomedical Engineering Scaffolds providing a 3D environment which can effectively promote the adhesion, proliferation and differentiation of cells are crucial to tissue regeneration. In this study, the poly-l-lactic acid (PLLA) scaffold with hierarchical pore structural was fabricated via two-step thermally induced phase separation (TIPS). To mimic both physical architecture and chemical composite of natural bone extracellular matrix (ECM), gelatin fibers were introduced into the pores of PLLA scaffolds and formed 3D network structure via TIPS. Human adipose tissue-derived stem cells (ADSCs) were harvested and seeded into PLLA/gel hybrid scaffolds and cultured in vitro for biocompatibility assay. The surface morphology, porosity and compressive modulus of scaffolds were characterized by scanning electron microscopy (SEM), density analysis and compression test respectively. The results showed that hybrid scaffolds had high porosity (91.62%), a good compressive modulus (2.79 ± 0.20 MPa), nanometer fibers (diameter around 186.39~354.30 nm) and different grades of pore size from 7.41 ± 2.64 nm to 387.94 ± 102.48 nm. The scaffolds with mild hydrolysis by NaOH were modified by 1-ethyl-3-(3-dimethyl ami-nopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). Gelatin was performed onto PLLA scaffold via TIPS aiming at enhancement cell-material interaction. In comparison with PLLA scaffold, the PLLA/gel scaffold had better biological performance and the mechanical properties because the gelatin fibers homogeneously distributed in each pore of PLLA scaffold and formed 3D network structure. Published version 2015-09-23T03:31:13Z 2019-12-06T21:10:56Z 2015-09-23T03:31:13Z 2019-12-06T21:10:56Z 2015 2015 Journal Article Song, K., Ji, L., Zhang, J., Wang, H., Jiao, Z., Mayasari, L., et al. (2015). Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure. Nanomaterials, 5(2), 415-424. 2079-4991 https://hdl.handle.net/10356/103362 http://hdl.handle.net/10220/38731 10.3390/nano5020415 en Nanomaterials © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
description Scaffolds providing a 3D environment which can effectively promote the adhesion, proliferation and differentiation of cells are crucial to tissue regeneration. In this study, the poly-l-lactic acid (PLLA) scaffold with hierarchical pore structural was fabricated via two-step thermally induced phase separation (TIPS). To mimic both physical architecture and chemical composite of natural bone extracellular matrix (ECM), gelatin fibers were introduced into the pores of PLLA scaffolds and formed 3D network structure via TIPS. Human adipose tissue-derived stem cells (ADSCs) were harvested and seeded into PLLA/gel hybrid scaffolds and cultured in vitro for biocompatibility assay. The surface morphology, porosity and compressive modulus of scaffolds were characterized by scanning electron microscopy (SEM), density analysis and compression test respectively. The results showed that hybrid scaffolds had high porosity (91.62%), a good compressive modulus (2.79 ± 0.20 MPa), nanometer fibers (diameter around 186.39~354.30 nm) and different grades of pore size from 7.41 ± 2.64 nm to 387.94 ± 102.48 nm. The scaffolds with mild hydrolysis by NaOH were modified by 1-ethyl-3-(3-dimethyl ami-nopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). Gelatin was performed onto PLLA scaffold via TIPS aiming at enhancement cell-material interaction. In comparison with PLLA scaffold, the PLLA/gel scaffold had better biological performance and the mechanical properties because the gelatin fibers homogeneously distributed in each pore of PLLA scaffold and formed 3D network structure.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Song, Kedong
Ji, Lili
Zhang, Jingying
Wang, Hai
Jiao, Zeren
Mayasari, Lim
Fu, Xiaoyan
Liu, Tianqing
format Article
author Song, Kedong
Ji, Lili
Zhang, Jingying
Wang, Hai
Jiao, Zeren
Mayasari, Lim
Fu, Xiaoyan
Liu, Tianqing
spellingShingle Song, Kedong
Ji, Lili
Zhang, Jingying
Wang, Hai
Jiao, Zeren
Mayasari, Lim
Fu, Xiaoyan
Liu, Tianqing
Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure
author_sort Song, Kedong
title Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure
title_short Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure
title_full Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure
title_fullStr Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure
title_full_unstemmed Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure
title_sort fabrication and cell responsive behavior of macroporous plla/gelatin composite scaffold with hierarchical micro-nano pore structure
publishDate 2015
url https://hdl.handle.net/10356/103362
http://hdl.handle.net/10220/38731
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