The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia

Resistance to pathogens is important for the sustainability and profitability of food fish production. In immune-related genes, the lipopolysaccharide-binding protein (LBP) gene is an important mediator of the inflammatory reaction. We analyzed the cDNA and genomic structure of the LBP gene in tilap...

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Main Authors: Xia, Jun Hong, Fu, Gui Hong, Liu, Feng, Yue, Gen Hua
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/103373
http://hdl.handle.net/10220/24472
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1033732023-02-28T17:05:38Z The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia Xia, Jun Hong Fu, Gui Hong Liu, Feng Yue, Gen Hua School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology Resistance to pathogens is important for the sustainability and profitability of food fish production. In immune-related genes, the lipopolysaccharide-binding protein (LBP) gene is an important mediator of the inflammatory reaction. We analyzed the cDNA and genomic structure of the LBP gene in tilapia. The full-length cDNA (1901 bp) of the gene contained a 1416 bp open reading frame, encoding 471 amino acid residues. Its genomic sequence was 5577 bp, comprising 15 exons and 14 introns. Under normal conditions, the gene was constitutively expressed in all examined tissues. The highest expression was detected in intestine and kidney. We examined the responses of the gene to challenges with two bacterial pathogens Streptcoccus agalactiae and Aeromonas hydrophila. The gene was significantly upregulated in kidney and spleen post-infection with S. agalactiae and A. hydrophila, respectively. However, the expression profiles of the gene after the challenge with the two pathogens were different. Furthermore, we identified three SNPs in the gene. There were significant associations (p < 0.05) of two of the three SNPs with the resistance to A. hydrophila, but not with the resistance to S. agalactiae or growth performance. These results suggest that the LBP gene is involved in the acute-phase immunologic response to the bacterial infections, and the responses to the two bacterial pathogens are different. The two SNPs associated with the resistance to A. hydrophila may be useful in the selection of tilapia resistant to A. hydrophila. Published version 2014-12-17T01:25:45Z 2019-12-06T21:11:12Z 2014-12-17T01:25:45Z 2019-12-06T21:11:12Z 2014 2014 Journal Article Fu, G. H., Liu, F., Xia, J. H., & Yue, G. H. (2014). The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia. International journal of molecular sciences, 15(12), 22028-22041. 1422-0067 https://hdl.handle.net/10356/103373 http://hdl.handle.net/10220/24472 10.3390/ijms151222028 25470022 en International journal of molecular sciences © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). 10 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Molecular biology
spellingShingle DRNTU::Science::Biological sciences::Molecular biology
Xia, Jun Hong
Fu, Gui Hong
Liu, Feng
Yue, Gen Hua
The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia
description Resistance to pathogens is important for the sustainability and profitability of food fish production. In immune-related genes, the lipopolysaccharide-binding protein (LBP) gene is an important mediator of the inflammatory reaction. We analyzed the cDNA and genomic structure of the LBP gene in tilapia. The full-length cDNA (1901 bp) of the gene contained a 1416 bp open reading frame, encoding 471 amino acid residues. Its genomic sequence was 5577 bp, comprising 15 exons and 14 introns. Under normal conditions, the gene was constitutively expressed in all examined tissues. The highest expression was detected in intestine and kidney. We examined the responses of the gene to challenges with two bacterial pathogens Streptcoccus agalactiae and Aeromonas hydrophila. The gene was significantly upregulated in kidney and spleen post-infection with S. agalactiae and A. hydrophila, respectively. However, the expression profiles of the gene after the challenge with the two pathogens were different. Furthermore, we identified three SNPs in the gene. There were significant associations (p < 0.05) of two of the three SNPs with the resistance to A. hydrophila, but not with the resistance to S. agalactiae or growth performance. These results suggest that the LBP gene is involved in the acute-phase immunologic response to the bacterial infections, and the responses to the two bacterial pathogens are different. The two SNPs associated with the resistance to A. hydrophila may be useful in the selection of tilapia resistant to A. hydrophila.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Xia, Jun Hong
Fu, Gui Hong
Liu, Feng
Yue, Gen Hua
format Article
author Xia, Jun Hong
Fu, Gui Hong
Liu, Feng
Yue, Gen Hua
author_sort Xia, Jun Hong
title The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia
title_short The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia
title_full The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia
title_fullStr The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia
title_full_unstemmed The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia
title_sort lbp gene and its association with resistance to aeromonas hydrophila in tilapia
publishDate 2014
url https://hdl.handle.net/10356/103373
http://hdl.handle.net/10220/24472
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