Bacterial metabolism during biofilm growth investigated by 13C tracing

• Main Authors: Wan, Ni, Wang, Hao, Ng, Chun Kiat, Mukherjee, Manisha, Ren, Dacheng, Cao, Bin, Tang, Yinjie J.
• Other Authors: School of Civil and Environmental Engineering
• Format: Article
• Language: English
• Published: 2019
• Subjects: Dynamic Labeling; Entner-Doudoroff Pathway; DRNTU::Engineering::Environmental engineering
• Online Access: https://hdl.handle.net/10356/103476; http://hdl.handle.net/10220/47325
• Institution: Nanyang Technological University

This study investigated the metabolism of Pseudomonas aeruginosa PAO1 during its biofilm development via microscopy imaging, gene expression analysis, and 13C-labeling. First, dynamic labeling was employed to investigate glucose utilization rate in fresh biofilms (thickness 40∼60 micrometer). The la...