A microfluidic co-culture system to monitor tumor-stromal interactions on a chip

The living cells are arranged in a complex natural environment wherein they interact with extracellular matrix and other neighboring cells. Cell-cell interactions, especially those between distinct phenotypes, have attracted particular interest due to the significant physiological relevance they can...

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Main Authors: Menon, Nishanth V., Chuah, Yon Jin, Cao, Bin, Lim, Mayasari, Kang, Yuejun
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/103579
http://hdl.handle.net/10220/24555
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1035792022-02-16T16:30:09Z A microfluidic co-culture system to monitor tumor-stromal interactions on a chip Menon, Nishanth V. Chuah, Yon Jin Cao, Bin Lim, Mayasari Kang, Yuejun School of Chemical and Biomedical Engineering School of Civil and Environmental Engineering Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences::Microbiology The living cells are arranged in a complex natural environment wherein they interact with extracellular matrix and other neighboring cells. Cell-cell interactions, especially those between distinct phenotypes, have attracted particular interest due to the significant physiological relevance they can reveal for both fundamental and applied biomedical research. To study cell-cell interactions, it is necessary to develop co-culture systems, where different cell types can be cultured within the same confined space. Although the current advancement in lab-on-a-chip technology has allowed the creation of in vitro models to mimic the complexity of in vivo environment, it is still rather challenging to create such co-culture systems for easy control of different colonies of cells. In this paper, we have demonstrated a straightforward method for the development of an on-chip co-culture system. It involves a series of steps to selectively change the surface property for discriminative cell seeding and to induce cellular interaction in a co-culture region. Bone marrow stromal cells (HS5) and a liver tumor cell line (HuH7) have been used to demonstrate this co-culture model. The cell migration and cellular interaction have been analyzed using microscopy and biochemical assays. This co-culture system could be used as a disease model to obtain biological insight of pathological progression, as well as a tool to evaluate the efficacy of different drugs for pharmaceutical studies. Published version 2014-12-26T07:44:56Z 2019-12-06T21:15:51Z 2014-12-26T07:44:56Z 2019-12-06T21:15:51Z 2014 2014 Journal Article Menon, N. V., Chuah, Y. J., Cao, B., Lim, M., & Kang, Y. (2014). A microfluidic co-culture system to monitor tumor-stromal interactions on a chip. Biomicrofluidics, 8(6), 064118-. 1932-1058 https://hdl.handle.net/10356/103579 http://hdl.handle.net/10220/24555 10.1063/1.4903762 25553194 en Biomicrofluidics © 2014 AIP Publishing LLC. This paper was published in Biomicrofluidics and is made available as an electronic reprint (preprint) with permission of AIP Publishing LLC. The paper can be found at the following official DOI: [http://dx.doi.org/10.1063/1.4903762]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 12 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology
spellingShingle DRNTU::Science::Biological sciences::Microbiology
Menon, Nishanth V.
Chuah, Yon Jin
Cao, Bin
Lim, Mayasari
Kang, Yuejun
A microfluidic co-culture system to monitor tumor-stromal interactions on a chip
description The living cells are arranged in a complex natural environment wherein they interact with extracellular matrix and other neighboring cells. Cell-cell interactions, especially those between distinct phenotypes, have attracted particular interest due to the significant physiological relevance they can reveal for both fundamental and applied biomedical research. To study cell-cell interactions, it is necessary to develop co-culture systems, where different cell types can be cultured within the same confined space. Although the current advancement in lab-on-a-chip technology has allowed the creation of in vitro models to mimic the complexity of in vivo environment, it is still rather challenging to create such co-culture systems for easy control of different colonies of cells. In this paper, we have demonstrated a straightforward method for the development of an on-chip co-culture system. It involves a series of steps to selectively change the surface property for discriminative cell seeding and to induce cellular interaction in a co-culture region. Bone marrow stromal cells (HS5) and a liver tumor cell line (HuH7) have been used to demonstrate this co-culture model. The cell migration and cellular interaction have been analyzed using microscopy and biochemical assays. This co-culture system could be used as a disease model to obtain biological insight of pathological progression, as well as a tool to evaluate the efficacy of different drugs for pharmaceutical studies.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Menon, Nishanth V.
Chuah, Yon Jin
Cao, Bin
Lim, Mayasari
Kang, Yuejun
format Article
author Menon, Nishanth V.
Chuah, Yon Jin
Cao, Bin
Lim, Mayasari
Kang, Yuejun
author_sort Menon, Nishanth V.
title A microfluidic co-culture system to monitor tumor-stromal interactions on a chip
title_short A microfluidic co-culture system to monitor tumor-stromal interactions on a chip
title_full A microfluidic co-culture system to monitor tumor-stromal interactions on a chip
title_fullStr A microfluidic co-culture system to monitor tumor-stromal interactions on a chip
title_full_unstemmed A microfluidic co-culture system to monitor tumor-stromal interactions on a chip
title_sort microfluidic co-culture system to monitor tumor-stromal interactions on a chip
publishDate 2014
url https://hdl.handle.net/10356/103579
http://hdl.handle.net/10220/24555
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