A mariner transposon vector adapted for mutagenesis in oral streptococci

• Main Authors: Nilsson, Martin, Christiansen, Natalia, Høiby, Niels, Twetman, Svante, Givskov, Michael, Tolker-Nielsen, Tim
• Format: Article
• Language: English
• Published: 2014
• Subjects: Biofilms
• Online Access: https://hdl.handle.net/10356/103603; http://hdl.handle.net/10220/20017
• Institution: Nanyang Technological University

This article describes the construction and characterization of a mariner-based transposon vector designed for use in oral streptococci, but with a potential use in other Gram-positive bacteria. The new transposon vector, termed pMN100, contains the temperature-sensitive origin of replication repATs...