Mimicking cellular transport mechanism in stem cells through endosomal escape of new peptide-coated quantum dots
Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic...
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| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/103727 http://hdl.handle.net/10220/19284 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Protein transport is an important phenomenon in biological systems. Proteins are transported via several
mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is
the microtubule mediated protein transport. Up to now, there are no reports on synthetic systems
mimicking the biological protein transport mechanism. Here we report a highly efficient method of
mimicking the microtubule mediated protein transport using newly designed biotinylated peptides
encompassing a microtubule-associated sequence (MTAS) and a nuclear localization signaling (NLS)
sequence, and their final conjugation with streptavidin-coated CdSe/ZnS quantum dots (QDs). Our results
demonstrate that these novel bio-conjugated QDs enhance the endosomal escape and promote targeted
delivery into the nucleus of human mesenchymal stem cells via microtubules. Mimicking the cellular
transport mechanism in stem cells is highly desirable for diagnostics, targeting and therapeutic applications,
opening up new avenues in the area of drug delivery. |
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