A rapid and label-free SERS detection method for biomarkers in clinical biofluids

• Main Authors: Kong, Kien Voon, Leong, Weng Kee, Lam, Zhiyong, Gong, Tianxun, Goh, Douglas, Lau, Weber Kam On, Olivo, Malini
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Chemistry::Biochemistry
• Online Access: https://hdl.handle.net/10356/104002; http://hdl.handle.net/10220/24577
• Institution: Nanyang Technological University

A metal carbonyl-functionalized nanostructured substrate can be used in a rapid and simple assay for the detection of A1AT, a potential biomarker for bladder cancer, in clinical urine samples. The assay involves monitoring changes in the carbonyl stretching vibrations of the metal carbonyl via surface-enhanced Raman spectroscopy (SERS). These vibrations lie in the absorption spectral window of 1800–2200 cm−1, which is free of any spectral interference from biomolecules.