Zinc-substituted hydroxyapatite : a biomaterial with enhanced bioactivity and antibacterial properties

Hydroxyapatite (HA) is a synthetic biomaterial and has been found to promote new bone formation when implanted in a bone defect site. However, its use is often limited due to its slow osteointegration rate and low antibacterial activity, particularly where HA has to be used for long term biomedical...

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Main Authors: Thian, E. S., Konishi, T., Kawanobe, Y., Lim, P. N., Ho, B., Aizawa, M., Choong, Cleo Swee Neo
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/104641
http://hdl.handle.net/10220/16563
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1046412020-06-01T10:26:50Z Zinc-substituted hydroxyapatite : a biomaterial with enhanced bioactivity and antibacterial properties Thian, E. S. Konishi, T. Kawanobe, Y. Lim, P. N. Ho, B. Aizawa, M. Choong, Cleo Swee Neo School of Materials Science & Engineering DRNTU::Engineering::Materials Hydroxyapatite (HA) is a synthetic biomaterial and has been found to promote new bone formation when implanted in a bone defect site. However, its use is often limited due to its slow osteointegration rate and low antibacterial activity, particularly where HA has to be used for long term biomedical applications. This work will describe the synthesis and detailed characterization of zinc-substituted HA (ZnHA) as an alternative biomaterial to HA. ZnHA containing 1.6 wt% Zn was synthesized via a co-precipitation reaction between calcium hydroxide, orthophosphoric acid and zinc nitrate hexahydrate. Single-phase ZnHA particles with a rod-like morphology measuring ~50 nm in length and ~15 nm in width, were obtained and characterized using transmission electron microscopy and X-ray diffraction. The substitution of Zn into HA resulted in a decrease in both the a- and c-axes of the unit cell parameters, thereby causing the HA crystal structure to alter. In vitro cell culture work showed that ZnHA possessed enhanced bioactivity since an increase in the growth of human adipose-derived mesenchymal stem cells along with the bone cell differentiation markers, were observed. In addition, antibacterial work demonstrated that ZnHA exhibited antimicrobial capability since there was a significant decrease in the number of viable Staphylococcus aureus bacteria after in contact with ZnHA. 2013-10-17T06:55:00Z 2019-12-06T21:36:45Z 2013-10-17T06:55:00Z 2019-12-06T21:36:45Z 2013 2013 Journal Article Thian, E. S., Konishi, T., Kawanobe, Y., Lim, P. N., Choong, C. S. N., Ho, B., & Aizawa, M. (2013). Zinc-substituted hydroxyapatite : a biomaterial with enhanced bioactivity and antibacterial properties. Journal of materials science : materials in medicine, 24(2), 437-445. https://hdl.handle.net/10356/104641 http://hdl.handle.net/10220/16563 10.1007/s10856-012-4817-x en Journal of materials science : materials in medicine
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Materials
spellingShingle DRNTU::Engineering::Materials
Thian, E. S.
Konishi, T.
Kawanobe, Y.
Lim, P. N.
Ho, B.
Aizawa, M.
Choong, Cleo Swee Neo
Zinc-substituted hydroxyapatite : a biomaterial with enhanced bioactivity and antibacterial properties
description Hydroxyapatite (HA) is a synthetic biomaterial and has been found to promote new bone formation when implanted in a bone defect site. However, its use is often limited due to its slow osteointegration rate and low antibacterial activity, particularly where HA has to be used for long term biomedical applications. This work will describe the synthesis and detailed characterization of zinc-substituted HA (ZnHA) as an alternative biomaterial to HA. ZnHA containing 1.6 wt% Zn was synthesized via a co-precipitation reaction between calcium hydroxide, orthophosphoric acid and zinc nitrate hexahydrate. Single-phase ZnHA particles with a rod-like morphology measuring ~50 nm in length and ~15 nm in width, were obtained and characterized using transmission electron microscopy and X-ray diffraction. The substitution of Zn into HA resulted in a decrease in both the a- and c-axes of the unit cell parameters, thereby causing the HA crystal structure to alter. In vitro cell culture work showed that ZnHA possessed enhanced bioactivity since an increase in the growth of human adipose-derived mesenchymal stem cells along with the bone cell differentiation markers, were observed. In addition, antibacterial work demonstrated that ZnHA exhibited antimicrobial capability since there was a significant decrease in the number of viable Staphylococcus aureus bacteria after in contact with ZnHA.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Thian, E. S.
Konishi, T.
Kawanobe, Y.
Lim, P. N.
Ho, B.
Aizawa, M.
Choong, Cleo Swee Neo
format Article
author Thian, E. S.
Konishi, T.
Kawanobe, Y.
Lim, P. N.
Ho, B.
Aizawa, M.
Choong, Cleo Swee Neo
author_sort Thian, E. S.
title Zinc-substituted hydroxyapatite : a biomaterial with enhanced bioactivity and antibacterial properties
title_short Zinc-substituted hydroxyapatite : a biomaterial with enhanced bioactivity and antibacterial properties
title_full Zinc-substituted hydroxyapatite : a biomaterial with enhanced bioactivity and antibacterial properties
title_fullStr Zinc-substituted hydroxyapatite : a biomaterial with enhanced bioactivity and antibacterial properties
title_full_unstemmed Zinc-substituted hydroxyapatite : a biomaterial with enhanced bioactivity and antibacterial properties
title_sort zinc-substituted hydroxyapatite : a biomaterial with enhanced bioactivity and antibacterial properties
publishDate 2013
url https://hdl.handle.net/10356/104641
http://hdl.handle.net/10220/16563
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